血清白细胞介素-8 作为食管鳞癌潜在的诊断生物标志物。

Serum interleukin-8 as a potential diagnostic biomarker in esophageal squamous cell carcinoma.

机构信息

Shantou University Medical College, Shantou, Guangdong, China.

Department of Clinical Laboratory Medicine, The Cancer Hospital of Shantou University Medical College, Shantou, Guangdong, China.

出版信息

Cancer Biomark. 2020;29(1):139-149. doi: 10.3233/CBM-201687.

DOI:10.3233/CBM-201687

PMID:32623391

Abstract

BACKGROUND

Esophageal squamous cell carcinoma (ESCC) has poor prognosis mainly due to the difficulty of making early diagnosis. Therefore, novel biomarkers are critically needed.

OBJECTIVE

We aimed to investigate the diagnostic value of serum interleukin-8 (IL-8) in ESCC.

METHODS

Data mining of TCGA was used to analyze expression level of IL-8 mRNA in esophageal carcinoma. Serum levels of IL-8 were measured in 103 ESCC patients and 86 normal controls by ELISA. Receiver operating characteristic (ROC) curve was used to evaluate its diagnostic accuracy.

RESULTS

IL-8 mRNA expression level and serum IL-8 concentration were both statistically higher in patients than normal controls (P< 0.001). ROC curve demonstrated that the optimum diagnostic cut-off for serum IL-8 was 80.082 pg/mL, providing an area under the curve (AUC) of 0.694 (95% CI: 0.620-0.768), with specificity of 86.0% and sensitivity of 42.7%. The AUC for early-stage ESCC was 0.618 (95% CI: 0.499-0.737), with sensitivity of 35.3% and specificity of 86.0%. Kaplan-Meier analysis and the log-rank test indicated that IL-8 may not be a prognostic predictor for ESCC.

CONCLUSIONS

Serum IL-8 was highly expressed in ESCC patients and may be a potential marker for early diagnosis of ESCC.

摘要

背景

食管鳞状细胞癌（ESCC）预后较差，主要是由于早期诊断困难。因此，迫切需要新的生物标志物。

目的

本研究旨在探讨血清白细胞介素-8（IL-8）在 ESCC 中的诊断价值。

方法

利用 TCGA 数据挖掘分析食管癌中 IL-8mRNA 的表达水平。采用 ELISA 法检测 103 例 ESCC 患者和 86 例正常对照者血清 IL-8 水平。采用受试者工作特征（ROC）曲线评价其诊断准确性。

结果

患者 IL-8mRNA 表达水平和血清 IL-8 浓度均明显高于正常对照组（P<0.001）。ROC 曲线分析显示，血清 IL-8 的最佳诊断界值为 80.082pg/ml，曲线下面积（AUC）为 0.694（95%CI：0.620-0.768），特异性为 86.0%，敏感性为 42.7%。早期 ESCC 的 AUC 为 0.618（95%CI：0.499-0.737），敏感性为 35.3%，特异性为 86.0%。Kaplan-Meier 分析和对数秩检验表明，IL-8 可能不是 ESCC 的预后预测因子。