Alonso G, Czernichow P, Assenmacher I
Cell Tissue Res. 1985;240(2):375-80. doi: 10.1007/BF00222348.
The effects of reserpine on the osmotically induced release of pituitary vasopressin were studied (i) by measuring the urinary excretion and the vasopressin content of the neural lobe of pituitary, and (ii) by examining the ultrastructural morphology of axons in the neural lobe of dehydrated rats. After water deprivation for two days, control rats displayed characteristic antidiuretic response including a 75% reduction of urinary excretion and a six-fold decrease in vasopressin content of the neural lobe associated with a dramatic depletion of neurosecretory granules in corresponding axons. In contrast, when they received two dialy injections of reserpine, animals dehydrated for two days showed both urinary excretion and vasopressin contents in the neural lobe that remained at levels comparable to those measured in the normally hydrated rats. Additionally, neural-lobe axons of such dehydrated, reserpine-treated rats displayed a normal amount of neurosecretory granules. These data indicate that reserpine inhibits release of vasopressin from the neural lobe and favour the concept of a facilitatory role of the catecholaminergic innervation in the control of hypothalamo-neurohypophysial vasopressin-secreting neurons.
（i）测量尿排泄量以及垂体神经叶中抗利尿激素的含量；（ii）检查脱水大鼠神经叶中轴突的超微结构形态。在禁水两天后，对照大鼠表现出典型的抗利尿反应，包括尿排泄量减少75%以及神经叶中抗利尿激素含量降低6倍，同时相应轴突中的神经分泌颗粒显著减少。相比之下，当给予利血平每日两次注射时，脱水两天的动物神经叶中的尿排泄量和抗利尿激素含量均维持在与正常水合大鼠相当的水平。此外，经利血平处理的脱水大鼠的神经叶轴突显示出正常数量的神经分泌颗粒。这些数据表明，利血平抑制抗利尿激素从神经叶的释放，支持了儿茶酚胺能神经支配在控制下丘脑 - 神经垂体抗利尿激素分泌神经元中起促进作用的观点。
