Iversen J M, Kauffman D L, Keller P J, Robinovitch M
Cell Tissue Res. 1985;240(2):441-7. doi: 10.1007/BF00222357.
A method is described for the isolation of two populations of secretory granules from rat parotid glands utilizing differences in their sedimentation characteristics. The granule preparations were analyzed for homogeneity by electron microscopy and chemical analyses. The soluble contents of both types of granules were obtained by hypotonic lysis, and the proteins compared by SDS-PAGE and ion exchange-gel filtration chromatography. Both populations of secretory granules appear to have the same protein composition as that of the parotid saliva. The secretory granules with the smaller apparent buoyant density became labelled with radioactive leucine earlier than the heavier granules when a pulse of this amino acid was supplied to a gland slice system. The lighter granules appear to represent an earlier stage in maturation.
描述了一种利用大鼠腮腺分泌颗粒沉降特性的差异来分离两类分泌颗粒群体的方法。通过电子显微镜和化学分析对颗粒制剂的均一性进行了分析。通过低渗裂解获得两种类型颗粒的可溶性成分，并通过SDS - PAGE和离子交换 - 凝胶过滤色谱法比较蛋白质。两类分泌颗粒群体似乎都具有与腮腺唾液相同的蛋白质组成。当向腺体切片系统提供该氨基酸脉冲时，表观浮力密度较小的分泌颗粒比密度较大的颗粒更早地被放射性亮氨酸标记。较轻的颗粒似乎代表成熟的早期阶段。
