Robinovitch M R, Lagunoff D, Iversen J M
J Histochem Cytochem. 1980 Dec;28(12):1351-4. doi: 10.1177/28.12.7229338.
Rat parotid secretory granule preparations contain, in addition to the acinar secretory granules, a second type of granule. Whereas the acinar granules lyse under hypotonic conditions, this second type of granule does not, thus providing a means for obtaining a fraction sufficiently enriched in these granules to allow for their characterization. In the present study, these granules are shown to possess demonstrable chymotrypsin-like enzyme activity. In the intact rat parotid, such activity is shown by histochemical methods to be present in the numerous mast cells residing in the connective tissue stroma, but no such activity exists in any of the parenchymal cells. On the basis of their electron microscopic appearance, enzyme activity, and physical characteristics it is concluded that the second type of granule present in rat parotid secretory granule preparations originates from stromal mast cells rather than from parenchymal cells.
大鼠腮腺分泌颗粒制剂中，除了腺泡分泌颗粒外，还含有第二种类型的颗粒。腺泡颗粒在低渗条件下会溶解，而这种第二种类型的颗粒则不会，因此提供了一种获取足够富集这些颗粒的组分以对其进行表征的方法。在本研究中，这些颗粒显示出可证实的类胰凝乳蛋白酶活性。在完整的大鼠腮腺中，通过组织化学方法显示这种活性存在于结缔组织基质中的大量肥大细胞中，但实质细胞中不存在这种活性。根据它们的电子显微镜外观、酶活性和物理特性，可以得出结论，大鼠腮腺分泌颗粒制剂中存在的第二种类型的颗粒起源于基质肥大细胞而非实质细胞。
