Singh Indrajeet, Rao Santosh T R B, Irving Helen R, Balani Kantesh, Kong Ing
Advanced Polymer and Composite Materials Laboratory, Department of Engineering, School of Computing, Engineering and Mathematical Sciences, La Trobe University, Bendigo, VIC 3550, Australia; Department of Materials Science and Engineering, Indian Institute of Technology Kanpur (208016), India.
Advanced Polymer and Composite Materials Laboratory, Department of Engineering, School of Computing, Engineering and Mathematical Sciences, La Trobe University, Bendigo, VIC 3550, Australia.
Int J Biol Macromol. 2025 May;305(Pt 1):141106. doi: 10.1016/j.ijbiomac.2025.141106. Epub 2025 Feb 14.
Alginate-based hydrogels are promising biomaterials for extrusion-based bioprinting; however, their poor mechanical properties, printability, and shape integrity limit their utility in mimicking complex tissues and organs. In this study, a novel sodium alginate (Alg)/58S bioactive glass (BG)-based ink was developed for soft tissue engineering applications. The inks were characterised for shear-thinning, flowability, and shape integrity by printing various structures, including single filaments (0° and 90° nozzle movement), scaffolds, and rings. The ABG10 ink (10 wt% 58S BG in Alg) exhibited superior printability, achieving a printing accuracy of over 90 %, compared to a printing accuracy of 30-40 % for pure Alg. Fourier transform infrared spectroscopy revealed interactions between 58S BG and the Alg matrix, while scanning electron microscopy characterised the 58S BG morphology within the matrix. The storage modulus increased from 767 (pure Alg) to 13,604 Pa (ABG10), while compressive strength rose from 23 ± 3 to 43 ± 4 kPa (58 % enhancement). The cytocompatibility of the inks was assessed using an MTT assay (with SH-SY5Y cells), which confirmed that ABG10 ink supports cell viability. Overall, ABG10 hydrogel-based inks exhibited enhanced shear-thinning behaviour, printability, mechanical strength, and cytocompatibility, which could help to develop patient-specific soft tissues.
基于海藻酸盐的水凝胶是用于基于挤出的生物打印的有前途的生物材料；然而，它们较差的机械性能、可打印性和形状完整性限制了它们在模拟复杂组织和器官方面的应用。在本研究中，开发了一种新型的基于海藻酸钠（Alg）/58S生物活性玻璃（BG）的墨水用于软组织工程应用。通过打印各种结构，包括单丝（0°和90°喷嘴移动）、支架和环，对墨水的剪切变稀、流动性和形状完整性进行了表征。与纯Alg 30%-40%的打印精度相比，ABG10墨水（Alg中含10 wt% 58S BG）表现出优异的可打印性，打印精度超过90%。傅里叶变换红外光谱揭示了58S BG与Alg基质之间的相互作用，而扫描电子显微镜表征了基质内58S BG的形态。储能模量从767（纯Alg）增加到13,604 Pa（ABG10），而抗压强度从23±3 kPa增加到43±4 kPa（提高了58%）。使用MTT法（用SH-SY5Y细胞）评估了墨水的细胞相容性，结果证实ABG10墨水支持细胞活力。总体而言，基于ABG10水凝胶的墨水表现出增强的剪切变稀行为、可打印性、机械强度和细胞相容性，这有助于开发针对患者的软组织。
