两种不同方法对结构性血红蛋白变异患者的糖化血红蛋白A1C结果比较。
Comparison of hemoglobin A1C results by two different methods on patients with structural hemoglobin variants.
作者信息
Blakney G B, Higgins T N, Holmes D J
机构信息
Chemistry Department, Dynacare Kasper Medical Laboratories, Edmonton, Alberta, Canada.
出版信息
Clin Biochem. 1998 Nov;31(8):619-26. doi: 10.1016/s0009-9120(98)00076-9.
OBJECTIVES
The objective was to compare hemoglobin A1C (HbA1C) results obtained by two methods based on different analytical principles for individuals with a structural hemoglobin variant.
DESIGN AND METHODS
Hemoglobin A1C results were obtained using the Bio-Rad Variant (based on cation exchange chromatography) and the Bayer DCA 2000 (based on an immunological reaction) on individuals with a structural hemoglobin variant. The identity of the hemoglobin variant was confirmed by high pressure liquid chromatography (HPLC) and electrophoresis.
RESULTS
Hemoglobin A1C results obtained by the two methods on individuals with S, C, D, and E trait were in close agreement.
CONCLUSION
The Bio-Rad Variant and Bayer DCA 2000 produce equivalent hemoglobin A1C results on patients with S, C, and E trait. With appropriate correction, correlation of hemoglobin A1C results from the Bio-Rad Variant for individuals with D trait was good (r = 0.927). Glycohemoglobin results obtained by the two methods for some unusual structural hemoglobin variants were in close agreement.
目的
目的是比较基于不同分析原理的两种方法对具有结构血红蛋白变异体的个体所测得的糖化血红蛋白（HbA1C）结果。
设计与方法
使用伯乐Variant（基于阳离子交换色谱法）和拜耳DCA 2000（基于免疫反应）对具有结构血红蛋白变异体的个体进行糖化血红蛋白结果检测。通过高压液相色谱法（HPLC）和电泳确认血红蛋白变异体的身份。
结果
两种方法对具有S、C、D和E性状的个体所测得的糖化血红蛋白结果高度一致。
结论
伯乐Variant和拜耳DCA 2000对具有S、C和E性状的患者产生等效的糖化血红蛋白结果。经过适当校正后，伯乐Variant对具有D性状个体的糖化血红蛋白结果相关性良好（r = 0.927）。两种方法对一些不常见的结构血红蛋白变异体所测得的糖化血红蛋白结果高度一致。
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