BACKGROUND: This study explores the feasibility and effectiveness of ultrasound-guided acupoint injection at Neiguan acupoint to prevent postoperative nausea and vomiting (PONV) after laparoscopic cholecystectomy. METHODS: A total of 60 patients undergoing laparoscopic cholecystectomy in the General Surgery Department of Zhejiang Hospital from February 2021 to August 2021 were enrolled in this study. The patients were randomly divided into the injection group (n = 30) and the control group (n = 30). The patients in the injection group received ultrasound-guided injections of tropisetron at bilateral Neiguan acupoints, while those in the control group received intravenous injections of tropisetron. The incidence of PONV within 24 hours after surgery, the need for "rescue" treatment, the incidence of puncture complications, and patients' satisfaction with PONV management were evaluated. RESULT: Neiguan point can be accurately located by ultrasound guidance. Compared with the control group, the incidence of PONV was significantly decreased in the Injection group within 0 to 6 hours after surgery, and the vomiting rate was decreased within 6 to 12 hours after surgery (P < .05). CONCLUSION: Ultrasound-guided drug injection at Neiguan to prevent PONV has the characteristics of visualization and accuracy, which is easy to promote and use, and the effect is satisfactory.