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通过精确序列插入进行的碱基编辑恢复了水稻中隐性rc等位基因的功能。

Prime editing via precise sequence insertion restores function of the recessive rc allele in rice.

作者信息

Nguyen Cuong X, Nguyen Trong D, Dinh Thao T, Nguyen Linh T, Ly Linh K, Chu Ha H, La Thang C, Do Phat T

机构信息

Department of Plant Cell Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.

Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.

出版信息

Plant Cell Rep. 2025 Feb 17;44(3):57. doi: 10.1007/s00299-025-03450-9.

DOI:10.1007/s00299-025-03450-9
PMID:39961872
Abstract

An improved prime editing system precisely corrected a 14-bp deletion in the rc gene of white rice, restoring the production of brown pigments.

摘要

一种改进的碱基编辑系统精确校正了白米rc基因中的14个碱基对缺失,恢复了棕色色素的产生。

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Prime editing via precise sequence insertion restores function of the recessive rc allele in rice.通过精确序列插入进行的碱基编辑恢复了水稻中隐性rc等位基因的功能。
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引用本文的文献

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Prime Editing for Crop Improvement: A Systematic Review of Optimization Strategies and Advanced Applications.用于作物改良的碱基编辑:优化策略与先进应用的系统综述
Genes (Basel). 2025 Aug 16;16(8):965. doi: 10.3390/genes16080965.

本文引用的文献

1
Population genomic analysis unravels the evolutionary roadmap of pericarp color in rice.群体基因组分析揭示了水稻果皮颜色的进化路径。
Plant Commun. 2024 Mar 11;5(3):100778. doi: 10.1016/j.xplc.2023.100778. Epub 2023 Dec 6.
2
Generating herbicide resistant and dwarf rice germplasms through precise sequence insertion or replacement.通过精确的序列插入或替换来培育抗除草剂和矮化水稻种质。
Plant Biotechnol J. 2024 Feb;22(2):293-295. doi: 10.1111/pbi.14225. Epub 2023 Nov 22.
3
An engineered prime editor with enhanced editing efficiency in plants.
一种在植物中具有增强编辑效率的工程化先导编辑器。
Nat Biotechnol. 2022 Sep;40(9):1394-1402. doi: 10.1038/s41587-022-01254-w. Epub 2022 Mar 24.
4
Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing.利用双模板 Prime 编辑技术实现大片段 DNA 序列的可编程删除、替换、插入和倒位。
Nat Biotechnol. 2022 May;40(5):731-740. doi: 10.1038/s41587-021-01133-w. Epub 2021 Dec 9.
5
Search-and-replace genome editing without double-strand breaks or donor DNA.无双链断裂或供体 DNA 的搜索和替换基因组编辑。
Nature. 2019 Dec;576(7785):149-157. doi: 10.1038/s41586-019-1711-4. Epub 2019 Oct 21.
6
CRISPR/Cas9-mediated functional recovery of the recessive rc allele to develop red rice.利用 CRISPR/Cas9 介导的功能恢复隐性 rc 等位基因,开发红米。
Plant Biotechnol J. 2019 Nov;17(11):2096-2105. doi: 10.1111/pbi.13125. Epub 2019 May 7.
7
The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp.Rc和Rd基因参与水稻果皮中原花青素的合成。
Plant J. 2007 Jan;49(1):91-102. doi: 10.1111/j.1365-313X.2006.02958.x. Epub 2006 Dec 6.
8
Caught red-handed: Rc encodes a basic helix-loop-helix protein conditioning red pericarp in rice.当场抓获:Rc编码一种调控水稻红色果皮的碱性螺旋-环-螺旋蛋白。
Plant Cell. 2006 Feb;18(2):283-94. doi: 10.1105/tpc.105.038430. Epub 2006 Jan 6.