Institute of Crop Sciences (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
J Integr Plant Biol. 2018 Jul;60(7):536-540. doi: 10.1111/jipb.12650. Epub 2018 May 21.
Precise replacement of an existing allele in commercial cultivars with an elite allele is a major goal in crop breeding. A single nucleotide polymorphism in the NRT1.1B gene between japonica and indica rice is responsible for the improved nitrogen use efficiency in indica rice. Herein, we precisely replaced the japonica NRT1.1B allele with the indica allele, in just one generation, using CRISPR/Cas9 gene-editing technology. No additional selective pressure was needed to enrich the precise replacement events. This work demonstrates the feasibility of replacing any genes with elite alleles within one generation, greatly expanding our ability to improve agriculturally important traits.
在作物育种中，用优良等位基因精确替换商业品种中的现有等位基因是一个主要目标。在粳稻和籼稻之间的 NRT1.1B 基因中的一个单核苷酸多态性导致了籼稻中氮利用效率的提高。在此，我们使用 CRISPR/Cas9 基因编辑技术，仅在一代内就精确地将粳稻 NRT1.1B 等位基因替换为籼稻等位基因，而且不需要额外的选择压力来富集精确替换事件。这项工作表明，在一代内用优良等位基因替换任何基因的可行性，极大地扩展了我们改良农业重要性状的能力。
