聚焦于中枢神经系统的小鼠三维回转器模拟微重力效应的行为学和多组学分析

Behavioral and multiomics analysis of 3D clinostat simulated microgravity effect in mice focusing on the central nervous system.

作者信息

Zhou Li, Song Chenchen, Yang Hu, Zhao Lianlian, Li Xianglei, Sun Xiuping, Gao Kai, Guo Jianguo

机构信息

Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China.

出版信息

Sci Rep. 2025 Feb 17;15(1):5731. doi: 10.1038/s41598-025-90212-y.

DOI:10.1038/s41598-025-90212-y

PMID:39962314

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11833055/

Abstract

A study was conducted to evaluate the three-dimensional clinostat simulated microgravity effect on mouse models, focusing on the central nervous system. Eighteen mice were divided into three groups: control, survival box, and clinostat + survival box. Behavioral tests, femur micro-CT, brain transcriptomics, serum metabolomics, and fecal microbiomics were performed. Results showed decreased activity, altered gait, enhanced fear memory, bone loss, immune/endocrine changes in brain transcriptome, and altered metabolic pathways in serum and gut microbiota in clinostat-treated mice. The model closely mimics spaceflight-induced transcriptome changes, suggesting its value in studying microgravity-related neurological alterations and highlighting the need for attention to emotional changes in space.

摘要

进行了一项研究，以评估三维回转器模拟微重力对小鼠模型的影响，重点关注中枢神经系统。18只小鼠被分为三组：对照组、生存箱组和回转器+生存箱组。进行了行为测试、股骨微型计算机断层扫描、脑转录组学、血清代谢组学和粪便微生物组学研究。结果显示，经回转器处理的小鼠活动减少、步态改变、恐惧记忆增强、骨质流失、脑转录组中的免疫/内分泌变化以及血清和肠道微生物群中的代谢途径改变。该模型紧密模拟了太空飞行引起的转录组变化，表明其在研究微重力相关神经学改变方面的价值，并强调了关注太空情绪变化的必要性。