Mains R E, Myers A C, Eipper B A
Endocrinology. 1985 Jun;116(6):2505-15. doi: 10.1210/endo-116-6-2505.
The factors controlling levels of peptidyl glycine alpha-amidating monooxygenase (PAM) activity in its major tissue sources in the adult male rat were investigated by carrying out a variety of endocrine, pharmacological, and dietary manipulations. Levels of PAM activity and alpha MSH immunoactivity in the neurointermediate lobe of the pituitary gland rose and fell in parallel in rats treated with the dopamine antagonist haloperidol or the dopamine agonist bromocriptine, respectively. PAM activity in the anterior pituitary lobe was increased after adrenalectomy or castration and decreased after thyroidectomy or treatment with haloperidol. PAM activity in the submandibular gland was increased after treatment with the alpha-adrenergic antagonist phenoxybenzamine and decreased after treatment with the alpha-adrenergic agonist phenylephrine. Serum levels of PAM activity were unaltered after hypophysectomy, adrenalectomy, sialectomy, or castration, but rose after thyroidectomy and declined after treatment with the ganglionic blocker chlorisondamine or phenoxybenzamine. Chronic dietary copper deficiency in rats resulted in increased PAM activity in homogenates of anterior pituitary lobe and submandibular gland assayed under optimized conditions; chronic dietary ascorbate deficiency in guinea pigs did not produce consistent changes in PAM activity in the tissues examined.
通过进行各种内分泌、药理学和饮食干预，研究了成年雄性大鼠主要组织来源中控制肽基甘氨酸α-酰胺化单加氧酶（PAM）活性水平的因素。在用多巴胺拮抗剂氟哌啶醇或多巴胺激动剂溴隐亭治疗的大鼠中，垂体神经中间叶的PAM活性水平和α-MSH免疫活性分别平行上升和下降。肾上腺切除或去势后垂体前叶的PAM活性增加，甲状腺切除或用氟哌啶醇治疗后降低。用α-肾上腺素能拮抗剂酚苄明治疗后下颌下腺的PAM活性增加，用α-肾上腺素能激动剂去氧肾上腺素治疗后降低。垂体切除、肾上腺切除、唾液腺切除或去势后血清PAM活性水平未改变，但甲状腺切除后升高，用神经节阻滞剂氯异吲哚铵或酚苄明治疗后降低。大鼠长期饮食铜缺乏导致在优化条件下测定的垂体前叶和下颌下腺匀浆中PAM活性增加；豚鼠长期饮食抗坏血酸缺乏在检查的组织中未产生PAM活性的一致变化。
