Rajasekariah G R, Rickard M D, O'Donnell I J
Exp Parasitol. 1985 Jun;59(3):321-7. doi: 10.1016/0014-4894(85)90087-6.
Antigens were derived from hatched and activated oncospheres of Taenia pisiformis which had been separated from embryophoric debris by centrifugation on Percoll. Crude oncospheral antigen was prepared by freeze-thawing and sonication of oncospheres at 4 C, and a supernatant of crude antigen was collected following centrifugation at 100,000g. Other antigens tested were the supernatants collected after 100,000g centrifugation of crude antigen solubilized in Triton X-100, butanol, lithium diiodosalicylic acid, KCl, sodium dodecyl sulfate, or sodium deoxycholate. When groups of rabbits were immunized with the various antigens and challenged with T. pisiformis eggs, both sodium deoxycholate- and Triton X-100-solubilized antigens stimulated a level of protection similar to the crude antigen. All other antigens failed to stimulate significant protective immunity. When sodium deoxycholate-solubilized antigen was fractionated using high-performance liquid chromatography, the major host-protective components were in the fractions with molecular weight greater than 140,000. Levels of the enzyme, glutamate dehydrogenase (EC 1.4.1.2), in the serum of rabbits challenged with T. pisiformis eggs closely reflected the degree of liver damage caused by migrating larvae, and were not markedly elevated in those rabbits effectively immunized using the crude or sodium deoxycholate-solubilized antigens.
抗原来源于经Percoll离心从胚胎性碎片中分离出的豆状带绦虫孵化并激活的六钩蚴。粗制六钩蚴抗原通过在4℃对六钩蚴进行冻融和超声处理制备，100,000g离心后收集粗制抗原的上清液。测试的其他抗原是粗制抗原分别用Triton X-100、丁醇、二碘水杨酸锂、KCl、十二烷基硫酸钠或脱氧胆酸钠溶解后经100,000g离心收集的上清液。当用各种抗原免疫兔群并用豆状带绦虫卵攻击时，脱氧胆酸钠和Triton X-100溶解的抗原刺激产生的保护水平与粗制抗原相似。所有其他抗原均未能刺激产生显著的保护性免疫。当用高效液相色谱法对脱氧胆酸钠溶解的抗原进行分级分离时，主要的宿主保护性成分存在于分子量大于140,000的级分中。用豆状带绦虫卵攻击的兔血清中谷氨酸脱氢酶（EC 1.4.1.2）的水平密切反映了移行幼虫引起的肝损伤程度，在用粗制或脱氧胆酸钠溶解的抗原有效免疫的兔中该酶水平并未明显升高。
