Craig P S, Zumbuehl O
Department of Parasitology, Liverpool School of Tropical Medicine, England.
J Helminthol. 1988 Mar;62(1):58-62. doi: 10.1017/s0022149x00011226.
Rabbits were vaccinated once, by subcutaneous and intradermal injection, with sonicates of oncospheres (TpO) or conditioned media from in vitro maintained mature metacestodes (TpMcES) of Taenia pisiformis. Extracts were either incorporated into or mixed with unilamellar liposomes (reverse phase evaporative vesicles) or emulsified in Freund's Incomplete Adjuvant (FIA). Control groups received liposomes or FIA without antigen, or antigen preparation without adjuvant. Rabbits were challenged orally two weeks after vaccination with approximately 1500 eggs of T. pisiformis and necropsied eight weeks after challenge. A mean of 155 cysts was recovered from seven control rabbits. A 67% reduction in peritoneal cyst numbers was obtained in TpO-IFA vaccinated rabbits compared to 75% for the TpO-liposome entrapped group. The highest level of protection (86%) was obtained when TpO was mixed with but not entrapped in liposomes. Only 32% and 39% reduction in peritoneal cyst numbers was obtained after immunizing with the TpMcES preparation in liposomes or IFA respectively, however greater than 85% of peritoneal metacestodes were dead (necrotic or calcified) and suggests a different immune response than occurs after vaccination with oncosphere extracts. Specific anti-oncospheral or anti-metacestode ES antibody (IgG) responses at two weeks post vaccination were similar in rabbits immunized with liposome or IFA associated extracts.
通过皮下和皮内注射，用豆状带绦虫六钩蚴的超声裂解物（TpO）或体外培养的成熟囊尾蚴的条件培养基（TpMcES）对兔子进行一次免疫接种。提取物要么掺入单层脂质体（反相蒸发囊泡）中，要么与之混合，要么在弗氏不完全佐剂（FIA）中乳化。对照组接受不含抗原的脂质体或FIA，或不含佐剂的抗原制剂。在接种疫苗两周后，给兔子口服约1500个豆状带绦虫卵进行攻毒，并在攻毒八周后进行尸检。从7只对照兔子中平均回收了155个囊肿。与包封于脂质体的TpO组75%的降低率相比，接种TpO - IFA的兔子腹膜囊肿数量减少了67%。当TpO与脂质体混合但未包封时，获得了最高水平的保护（86%）。在用脂质体或IFA中的TpMcES制剂免疫后，腹膜囊肿数量分别仅减少了32%和39%，然而超过85%的腹膜囊尾蚴已死亡（坏死或钙化），这表明与用六钩蚴提取物接种后发生的免疫反应不同。在用脂质体或与IFA相关的提取物免疫的兔子中，接种疫苗两周后的特异性抗六钩蚴或抗囊尾蚴ES抗体（IgG）反应相似。
