Rojas E, Pollard H B, Heldman E
FEBS Lett. 1985 Jun 17;185(2):323-7. doi: 10.1016/0014-5793(85)80931-5.
The luminescent oxidation of luciferin has been used to monitor acetylcholine-induced ATP release from cultured bovine chromaffin cells. Acetylcholine (1-100 microM) evoked ATP release of up to 30% of the total cellular ATP. This secretion required external free calcium and could also be elicited by K+-induced membrane depolarization. The size of the cytosolic ATP compartment was estimated as 5% of the ATP in the cell by solubilising the cell membrane using digitonin (20 microM) or by application to the cells of brief pulses (2 microseconds) of high electric field (2000 V/cm). Blockers of the voltage-gated Ca2+ channel effectively blocked K+-induced ATP release, while the acetylcholine antagonists d-tubocurarine and beta-bungarotoxin inhibited the acetylcholine-induced release of ATP. These data support the concept that ATP is released together with the catecholamines by exocytosis of chromaffin granule contents.
荧光素的发光氧化已被用于监测乙酰胆碱诱导的培养牛嗜铬细胞中ATP的释放。乙酰胆碱（1 - 100微摩尔）引起的ATP释放量高达细胞总ATP的30%。这种分泌需要细胞外游离钙，并且也可由钾离子诱导的膜去极化引发。通过用洋地黄皂苷（20微摩尔）溶解细胞膜或对细胞施加短暂的高电场脉冲（2微秒，2000伏/厘米），估计胞质ATP池的大小为细胞中ATP的5%。电压门控钙通道阻滞剂有效地阻断了钾离子诱导的ATP释放，而乙酰胆碱拮抗剂d -筒箭毒碱和β -银环蛇毒素抑制了乙酰胆碱诱导的ATP释放。这些数据支持了ATP与儿茶酚胺通过嗜铬颗粒内容物的胞吐作用一起释放的概念。
