Phillips Robert A, Oh Seyun, Bach Svitlana V, Du Yufeng, Miller Ryan A, Kleinman Joel E, Hyde Thomas M, Hicks Stephanie C, Page Stephanie C, Martinowich Keri
Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
iScience. 2025 Jan 16;28(2):111820. doi: 10.1016/j.isci.2025.111820. eCollection 2025 Feb 21.
The lateral septum (LS) is a midline, subcortical structure that is a critical regulator of social behaviors. Mouse studies have identified molecularly distinct neuronal populations within the LS, which control specific facets of social behavior. Despite its known molecular heterogeneity in the mouse and critical role in regulating social behavior, comprehensive molecular profiling of the human LS has not been performed. Here, we conducted single-nucleus RNA sequencing (snRNA-seq) to generate transcriptomic profiles of the human LS and compared human LS profiles to recently collected mouse LS snRNA-seq datasets. Our analyses identified as a conserved molecular marker of the mouse and human LS, while is enriched only in the human LS. We also identify a distinct neuronal cell type marked by , the gene encoding the μ-opioid receptor. Together, these results highlight transcriptional heterogeneity of the human LS and identify robust marker genes for the human LS.
外侧隔区（LS）是一种位于中线的皮质下结构，是社会行为的关键调节因子。小鼠研究已经确定了LS内分子上不同的神经元群体，它们控制着社会行为的特定方面。尽管已知其在小鼠中具有分子异质性且在调节社会行为中起关键作用，但尚未对人类LS进行全面的分子分析。在这里，我们进行了单核RNA测序（snRNA-seq）以生成人类LS的转录组图谱，并将人类LS图谱与最近收集的小鼠LS snRNA-seq数据集进行比较。我们的分析确定 为小鼠和人类LS的保守分子标记，而 仅在人类LS中富集。我们还鉴定出一种由编码μ-阿片受体的基因 标记的独特神经元细胞类型。总之，这些结果突出了人类LS的转录异质性，并确定了人类LS的可靠标记基因。
