Dai Huang, Yu Junlan, Zhou Run, Hao Guijie, Qiao Zhaohui, Feng Zhe, Liu Xin, Bi Jie, Wang Jiahua, Liu Xiaodan, Pi Fuwei, Shen Yafang
Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education; Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs; Huzhou Key Laboratory of Aquatic Product Quality Improvement and Processing Technology, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China.
Food Chem. 2025 Jun 1;476:143378. doi: 10.1016/j.foodchem.2025.143378. Epub 2025 Feb 11.
In this study, Pt/Au nanoparticles functionalized metal-organic frameworks (Pt/Au/MIL-100(Fe)) nanocomposite was employed as a nanozyme and a colorimetric aptasensor based on aptamer-modulated nanozymatic activity was developed for deoxynivalenol (DON) detection. The catalytic activity of Pt/Au/MIL-100(Fe) was initially inhibited by aptamers due to the blocking of surface-active sites. However, the presence of DON detached the aptamer from Pt/Au/MIL-100(Fe) surface, restoring the enzyme-mimicking activity of the nanocomposites and producing a measurable colorimetric signal for DON quantitation. The nanozyme showed stable catalytic activities with a relative standard deviation within 3 % for storage at 4 °C for 21 days. Meanwhile, the developed aptasensor demonstrated a good linear range of 50-5000 ng/mL with a limit of detection of 44.14 ng/mL. The recovery rates for detecting DON in positive wheat and maize flour samples were 98.59 %-106.27 %. The developed method is cost-effective and easy to fabricate, providing a powerful tool for DON detection in food.
在本研究中，采用铂/金纳米颗粒功能化的金属有机框架（Pt/Au/MIL-100(Fe)）纳米复合材料作为纳米酶，并基于适配体调节的纳米酶活性开发了一种用于脱氧雪腐镰刀菌烯醇（DON）检测的比色适配体传感器。由于表面活性位点被阻断，Pt/Au/MIL-100(Fe)的催化活性最初受到适配体的抑制。然而，DON的存在使适配体从Pt/Au/MIL-100(Fe)表面脱离，恢复了纳米复合材料的类酶活性，并产生可测量的比色信号用于DON定量。该纳米酶在4℃下储存21天，催化活性稳定，相对标准偏差在3%以内。同时，所开发的适配体传感器显示出50 - 5000 ng/mL的良好线性范围，检测限为44.14 ng/mL。在阳性小麦和玉米粉样品中检测DON的回收率为98.59% - 106.27%。所开发的方法具有成本效益且易于制备，为食品中DON的检测提供了有力工具。
