Abou-Samra M, Venema K, Ayoub Moubareck C, Karavetian M
Faculty of Health, Medicine and Life Sciences, School of Nutrition and Translational Research in Metabolism (NUTRIM), 385783Maastricht University, 6200 MD Maastricht, the Netherlands.
Centre for Healthy Eating and Food Innovation, Faculty of Science and Engineering, Maastricht University, Campus Venlo, 5928 SZ Venlo, the Netherlands.
Benef Microbes. 2025 Feb 17;16(4):395-411. doi: 10.1163/18762891-bja00062.
An altered gut microbiota has been shown to contribute to the development of metabolic diseases such as obesity. In this study gut microbiota profile of 30 obese and 23 lean Lebanese individuals was performed via DNA isolation and sequencing of the V3-V4 region of the 16S rRNA of faecal samples. The abundance of the phylum Verrucomicrobiota was higher in lean subjects and there was no significant difference in the Bacillota/ Bacteroidota ratio between the obese and lean groups. The evenness and Shannon alpha diversity indices were significantly higher in the lean group than in the obese group ( q = 0.012 and q = 0.030, respectively). Beta diversity was higher in the obese group based for unweighted uniFrac distance variability ( q = 0.047). Lachnoclostridium was the only genus that was higher in obese ( q = 0.013) and it is linked to diet induced obesity, while the abundance of the genera Peptococcus, Ruminococcus_2, Lachnospiraceae UCG-001, Ruminiclostridium 6, the uncharacterised taxon within Coriobacteriaceae, Ruminococcaceae UCG-005, Ruminococcaceae UCG-010 and Oxalobacter, were significantly higher in lean subjects. These bacterial species that were higher in lean people, possess anti-inflammatory properties through the production of short chain fatty acids and are linked with lower body mass index, promote satiety and weight loss and may play a role in the protection against obesity and type 2 diabetes. Further research to generate a clear understanding of the interaction of the gut microbiota and health is needed.
肠道微生物群的改变已被证明会导致肥胖等代谢性疾病的发生。在本研究中，通过对粪便样本16S rRNA的V3-V4区域进行DNA分离和测序，分析了30名肥胖和23名消瘦黎巴嫩人的肠道微生物群特征。疣微菌门在消瘦受试者中的丰度较高，肥胖组和消瘦组之间的厚壁菌门/拟杆菌门比率没有显著差异。消瘦组的均匀度和香农α多样性指数显著高于肥胖组（分别为q = 0.012和q = 0.030）。基于未加权单因素距离变异性，肥胖组的β多样性更高（q = 0.047）。瘤胃梭菌属是肥胖组中唯一丰度较高的属（q = 0.013），它与饮食诱导的肥胖有关，而消化球菌属、瘤胃球菌属_2、毛螺菌科UCG-001、瘤胃杆菌属6、放线菌科内未鉴定的分类群、瘤胃球菌科UCG-005、瘤胃球菌科UCG-010和草酸杆菌属在消瘦受试者中的丰度显著更高。这些在消瘦人群中丰度较高的细菌物种通过产生短链脂肪酸具有抗炎特性，并与较低的体重指数相关，促进饱腹感和体重减轻，可能在预防肥胖和2型糖尿病中发挥作用。需要进一步研究以清楚了解肠道微生物群与健康之间的相互作用。
