Bagheshzadeh Parisa, Amini Elaheh, Baniasadi Farzaneh, Tavana Somayeh, Mohammadikish Maryam
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Reprod Sci. 2025 Apr;32(4):1241-1261. doi: 10.1007/s43032-025-01816-8. Epub 2025 Feb 19.
An effective approach to enrich the in vitro maturation (IVM) of oocyte medium as one of the main assisted reproduction technologies is the use of antioxidants to minimize oxidative stress. This study examined the effects of copper nanoparticles (CuNPs) synthesized by chemical (Ch-NPs) and green (G-CuNPs) methods on the IVM process of mouse oocytes and the development of the embryo in comparison to control oocytes (without nanoparticles treatment). Hydroalcoholic (G-H-CuNPs) and aqueous (G-A-CuNPs) Rosmarinus officinalis extracts were used for green synthesis. Here, Ch-NPs showed much less nuclear maturation and survival rate (44.92 ± 4.52; 66.21 ± 6.22) than the control (73.36 ± 7.40; 89.33 ± 4.40), respectively (P < 0.001). In contrast, G-H-CuNPs treated oocytes exhibited a significant increase (72.28 ± 5.51; 79.37 ± 6.29) compared to the Ch-NPs (P < 0.05). The level of ROS in Ch-NPs exposed oocytes was significantly higher than in the control (P < 0.001). The fertilization rate exhibited a significant elevation in the G-H-CuNPs (96.00 ± 2.45) compared to the control (71.14 ± 5.20) and Ch-NPs (50.00 ± 0.01) (P < 0.05). The 8-cell and blastocyst (BL) rates in the G-H-CuNPs (70.32 ± 3.78) revealed notably higher than those in the control (64.29 ± 3.69) and Ch-NPs (36.67 ± 10.22) (P < 0.05). In summary, results exhibited that G-CuNPs promote mouse oocyte maturation, fertilization, and embryo development more than Ch-NPs. The follow-up studies propose looking into the safety and applicability of green-synthesized CuNPs in human-assisted reproductive technologies.
作为主要辅助生殖技术之一，丰富卵母细胞体外成熟（IVM）培养基的一种有效方法是使用抗氧化剂来最小化氧化应激。本研究检测了通过化学方法（Ch-NPs）和绿色方法（G-CuNPs）合成的铜纳米颗粒对小鼠卵母细胞IVM过程及胚胎发育的影响，并与对照卵母细胞（未进行纳米颗粒处理）进行比较。水醇提取物（G-H-CuNPs）和水提取物（G-A-CuNPs）的迷迭香用于绿色合成。在此，Ch-NPs的核成熟率和存活率（分别为44.92±4.52；66.21±6.22）远低于对照组（分别为73.36±7.40；89.33±4.40）（P<0.001）。相比之下，与Ch-NPs处理的卵母细胞相比，G-H-CuNPs处理的卵母细胞显著增加（72.28±5.51；79.37±6.29）（P<0.05）。Ch-NPs处理的卵母细胞中的活性氧水平显著高于对照组（P<0.001）。与对照组（71.14±5.20）和Ch-NPs（50.00±0.01）相比，G-H-CuNPs的受精率显著提高（96.00±2.45）（P<0.05）。G-H-CuNPs的8细胞和囊胚（BL）率（70.32±3.78）显著高于对照组（64.29±3.69）和Ch-NPs（36.67±10.22）（P<0.05）。总之，结果表明，与Ch-NPs相比，G-CuNPs更能促进小鼠卵母细胞成熟、受精和胚胎发育。后续研究建议探讨绿色合成的CuNPs在人类辅助生殖技术中的安全性和适用性。
