Thomas Alyssa R, Lazelle Kristen, Garcia Mancebo Julia, Romfh Padraic, Kheir John N
Division of Newborn Medicine, Department of Pediatrics, Brigham & Women's Hospital, 75 Francis St, Boston, MA, 02115, USA.
Division of Newborn Medicine, Department of Pediatrics, Boston Children's Hospital, Boston, MA, USA.
J Cardiovasc Transl Res. 2025 Feb 19. doi: 10.1007/s12265-025-10599-x.
Coarctation of the aorta (CoA) can cause aortic obstruction, ischemia, and death. Resonance Raman Spectroscopy (RRS) measures tissue oxyhemoglobin (ShbO) and mitochondrial redox state (3RMR) non-invasively. Metal wire was placed around the aorta of Sprague Dawley rats to generate a systolic blood pressure (SBP) gradient. RRS-ShbO and 3RMR were measured pre- (hand) and post-obstruction (foot). In model 1 (n = 8), the gradient rapidly reached 120 mmHg. In model 2 (n = 30), gradients of 20 mmHg (n = 9) and 40 mmHg (n = 12) were maintained for 2 h. In model 1, foot-ShbO and 3RMR changed significantly (P = 0.004 and P = 0.007) at SBP gradients of 80-mmHg or above. In model 2, the 40-mmHg gradient group showed significant declines in foot-ShbO2 (P = 0.014) and increases in 3RMR (P = 0.008) by 1 h. Foot-ShbO and 3RMR correlated strongly with serum mixed venous saturation (ShbO: r = 0.73, P < 0.0001; 3RMR: r = -0.55, P < 0.0001). RRS effectively detects ischemia caused by aortic obstruction in a CoA model.
主动脉缩窄（CoA）可导致主动脉梗阻、缺血和死亡。共振拉曼光谱（RRS）可无创测量组织氧合血红蛋白（ShbO）和线粒体氧化还原状态（3RMR）。将金属丝环绕在Sprague Dawley大鼠的主动脉周围以产生收缩压（SBP）梯度。在梗阻前（手部）和梗阻后（足部）测量RRS-ShbO和3RMR。在模型1（n = 8）中，梯度迅速达到120 mmHg。在模型2（n = 30）中，20 mmHg（n = 9）和40 mmHg（n = 12）的梯度维持2小时。在模型1中，当SBP梯度达到80 mmHg或以上时，足部ShbO和3RMR发生显著变化（P = 0.004和P = 0.007）。在模型2中，40 mmHg梯度组在1小时时足部ShbO2显著下降（P = 0.014），3RMR显著增加（P = 0.008）。足部ShbO和3RMR与血清混合静脉饱和度密切相关（ShbO：r = 0.73，P < 0.0001；3RMR：r = -0.55，P < 0.0001）。RRS可有效检测CoA模型中由主动脉梗阻引起的缺血。
