Wilhelm Léonore, Wang Yangzi, Xu Shuqing
Institute of Organismic and Molecular Evolution (iomE), Johannes Gutenberg University, 55128, Mainz, Germany.
Institute for Evolution and Biodiversity, University of Münster, 48161, Münster, Germany.
Sci Data. 2025 Feb 19;12(1):299. doi: 10.1038/s41597-025-04607-7.
The Colorado potato beetle (CPB) is a major pest of potato crops, known for its remarkable ability to develop resistance to more than 50 pesticides. For decades, CPB has served as a model species for studying insecticide resistance, insect physiology, diapause, reproduction, and evolution. However, research progress on CPB has been hindered by the lack of comprehensive genomic and transcriptomic resources. Here, leveraging a recently established chromosome-level genome assembly, we constructed a gene expression atlas of CPB using transcriptomic data from 61 samples representing major organs and developmental stages. By integrating short- and long-read sequencing technologies, we enhanced the genome annotation and identified 6,623 additional genes that were previously undetected. Furthermore, we developed a web portal to facilitate the search and visualization of the gene expression atlas, providing an accessible resource for the research community. The CPB gene expression atlas offers valuable tools and comprehensive data that will accelerate future research in pest control and insect biology.
科罗拉多马铃薯甲虫（CPB）是马铃薯作物的主要害虫，以其对50多种杀虫剂产生抗性的显著能力而闻名。几十年来，CPB一直是研究杀虫剂抗性、昆虫生理学、滞育、繁殖和进化的模式物种。然而，由于缺乏全面的基因组和转录组资源，CPB的研究进展受到了阻碍。在这里，利用最近建立的染色体水平基因组组装，我们使用来自代表主要器官和发育阶段的61个样本的转录组数据构建了CPB的基因表达图谱。通过整合短读长和长读长测序技术，我们改进了基因组注释，并鉴定出6623个以前未检测到的基因。此外，我们开发了一个门户网站，以促进基因表达图谱的搜索和可视化，为研究界提供了一个可访问的资源。CPB基因表达图谱提供了有价值的工具和全面的数据，将加速未来在害虫控制和昆虫生物学方面的研究。
