State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.
Sci Data. 2023 Jan 19;10(1):36. doi: 10.1038/s41597-023-01950-5.
The Colorado potato beetle (Leptinotarsa decemlineata) is one of the most notorious insect pests of potatoes globally. Here, we generated a high-quality chromosome-level genome assembly of L. decemlineata using a combination of the PacBio HiFi sequencing and Hi-C scaffolding technologies. The genome assembly (-1,008 Mb) is anchored to 18 chromosomes (17 + XO), with a scaffold N50 of 58.32 Mb. It contains 676 Mb repeat sequences and 29,606 protein-coding genes. The chromosome-level genome assembly of L. decemlineata provides in-depth knowledge and will be a helpful resource for the beetle and invasive biology research communities.
科罗拉多马铃薯甲虫(Leptinotarsa decemlineata)是全球马铃薯最著名的害虫之一。在这里,我们使用 PacBio HiFi 测序和 Hi-C 支架技术相结合,生成了 L. decemlineata 的高质量染色体水平基因组组装。基因组组装(-1008 Mb)被锚定到 18 条染色体(17 + XO)上,支架 N50 为 58.32 Mb。它包含 676 Mb 重复序列和 29606 个蛋白质编码基因。L. decemlineata 的染色体水平基因组组装提供了深入的知识,将成为甲虫和入侵生物学研究界的有用资源。