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《异色瓢虫 Leptinotarsa decemlineata 染色体水平基因组组装》

Chromosome-level genome assembly of the Colorado potato beetle, Leptinotarsa decemlineata.

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.

出版信息

Sci Data. 2023 Jan 19;10(1):36. doi: 10.1038/s41597-023-01950-5.

Abstract

The Colorado potato beetle (Leptinotarsa decemlineata) is one of the most notorious insect pests of potatoes globally. Here, we generated a high-quality chromosome-level genome assembly of L. decemlineata using a combination of the PacBio HiFi sequencing and Hi-C scaffolding technologies. The genome assembly (-1,008 Mb) is anchored to 18 chromosomes (17 + XO), with a scaffold N50 of 58.32 Mb. It contains 676 Mb repeat sequences and 29,606 protein-coding genes. The chromosome-level genome assembly of L. decemlineata provides in-depth knowledge and will be a helpful resource for the beetle and invasive biology research communities.

摘要

科罗拉多马铃薯甲虫(Leptinotarsa decemlineata)是全球马铃薯最著名的害虫之一。在这里,我们使用 PacBio HiFi 测序和 Hi-C 支架技术相结合,生成了 L. decemlineata 的高质量染色体水平基因组组装。基因组组装(-1008 Mb)被锚定到 18 条染色体(17 + XO)上,支架 N50 为 58.32 Mb。它包含 676 Mb 重复序列和 29606 个蛋白质编码基因。L. decemlineata 的染色体水平基因组组装提供了深入的知识,将成为甲虫和入侵生物学研究界的有用资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d591/9849343/e7b06887ee72/41597_2023_1950_Fig1_HTML.jpg

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