Brennan S O
Hemoglobin. 1985;9(1):53-63. doi: 10.3109/03630268508996982.
A high performance cation exchange system is described for isolating globin chains. The method is rapid and inexpensive, using total lysate, a 14 min gradient, and a 3 cm column. The running buffers contain 6 M urea to dissociate and unfold the globin, and a NaCl gradient is employed for elution. The procedure provides a convenient way of detecting mutant globins with an intrinsic alteration in their charge.
描述了一种用于分离珠蛋白链的高性能阳离子交换系统。该方法快速且成本低廉，使用总裂解物、14分钟的梯度和3厘米的柱子。运行缓冲液含有6M尿素以解离和展开珠蛋白，并采用NaCl梯度进行洗脱。该程序提供了一种检测电荷发生内在改变的突变珠蛋白的便捷方法。
