Sparham S J, Huehns E R
Hemoglobin. 1979;3(1):13-20. doi: 10.3109/03630267909069151.
A chromatographic procedure for the separation of human globin chains is described. This method uses CM-Sepharose CL-6B as ion-exchanger and NaCl gradients formed in sodium phosphate buffers containing urea and 0.05M mercaptoethanol to elute the chains. One advantage of this system is that a column, once packed, may be used repeatedly for several chain separations, thus reducing cost and practical work. The resolution in our hands was also better than that on CM-Cellulose using phosphate buffer gradients.
描述了一种分离人血红蛋白链的色谱方法。该方法使用CM-琼脂糖凝胶CL-6B作为离子交换剂，并用含尿素和0.05M巯基乙醇的磷酸钠缓冲液形成的NaCl梯度来洗脱各条链。该系统的一个优点是，一旦装填好柱子，可重复用于多次链分离，从而降低成本并减少实际工作量。在我们的操作中，其分辨率也优于使用磷酸盐缓冲液梯度的CM-纤维素柱。
