Garrel D R, Welsch C, Arnaud M J, Tourniaire J
Hum Nutr Clin Nutr. 1985 Jan;39(1):29-37.
Whole-body protein turnover was measured in eight women, using a single oral dose of 15N-glycine and urinary ammonia as the end product analysed. Energy and protein intakes were carefully controlled on the day of the measurement. Each subject was tested five or six times at weekly intervals. Four of the subjects were taking oral contraceptives, the other four were non-pill users. Values of whole-body protein turnover (flux) were within the range of previously reported data: 2.9-4.7 g/kg/d. Inter-individual variation was significantly greater than intra-individual variation. The latter ranged between 10 and 23 per cent. Protein turnover was significantly correlated with the serum T3/rT3 ratio (r = 0.845, P less than 0.01). No difference was found between pill users and non-pill users, nor between the luteal and the follicular phase of the menstrual cycle among non-pill users. These data suggest that the single-dose method with ammonia as the end product is suitable for human studies in clinical situations and that the phase of the menstrual cycle does not seem to be of critical importance when investigating menstruating women.
对8名女性进行了全身蛋白质周转率的测量，采用单次口服15N-甘氨酸，并分析尿氨作为终产物。在测量当天仔细控制能量和蛋白质摄入量。每个受试者每周接受五到六次测试。其中4名受试者正在服用口服避孕药，另外4名未服用避孕药。全身蛋白质周转率（通量）的值在先前报告的数据范围内：2.9 - 4.7克/千克/天。个体间差异显著大于个体内差异。个体内差异在10%至23%之间。蛋白质周转率与血清T3/rT3比值显著相关（r = 0.845，P < 0.01）。服用避孕药者与未服用避孕药者之间没有差异，未服用避孕药者的月经周期黄体期和卵泡期之间也没有差异。这些数据表明，以氨作为终产物的单剂量方法适用于临床情况下的人体研究，并且在调查月经周期女性时，月经周期阶段似乎并非至关重要。
