Weiss R E, Reddi A H
J Cell Biol. 1981 Mar;88(3):630-6. doi: 10.1083/jcb.88.3.630.
Fibronectin has been localized by indirect immunofluorescence during the various phases of endochondral bone formation in response to subcutaneously implanted demineralized bone matrix. Its histologic appearance has been correlated with results of biosynthetic experiments. (a) The implanted collagenous bone matrix was coated with fibronectin before and during mesenchymal cell proliferation. (b) During proliferation of mesenchymal precursor cells, the newly synthesized extracellular matrix exhibited a fibrillar network of fibronectin. (c) During cartilage differentiation, the fibronectin in the extracellular matrix was apparently masked by proteoglycans, as judged by hyaluronidase treatment. (d) Differentiating chondrocytes exhibited a uniform distribution of fibronectin. (e) Fibronectin was present in a cottony array around osteoblasts during osteogenesis. (f) The developing hematopoietic colonies revealed fibronectin associated with them. Therefore, it appears that fibronectin is ubiquitous throughout the development of endochondral bone and bone marrow.
在对皮下植入的脱矿骨基质的反应中，通过间接免疫荧光法对软骨内骨形成的各个阶段的纤连蛋白进行了定位。其组织学表现已与生物合成实验结果相关联。(a) 在间充质细胞增殖之前和期间，植入的胶原骨基质被纤连蛋白覆盖。(b) 在间充质前体细胞增殖期间，新合成的细胞外基质呈现出纤连蛋白的纤维状网络。(c) 在软骨分化期间，通过透明质酸酶处理判断，细胞外基质中的纤连蛋白显然被蛋白聚糖掩盖。(d) 分化中的软骨细胞呈现出纤连蛋白的均匀分布。(e) 在骨形成过程中，成骨细胞周围存在棉絮状排列的纤连蛋白。(f) 发育中的造血集落显示与其相关的纤连蛋白。因此，似乎纤连蛋白在软骨内骨和骨髓的整个发育过程中无处不在。
