纤连蛋白在软骨、骨和骨髓分化过程中的表现。
Appearance of fibronectin during the differentiation of cartilage, bone, and bone marrow.
作者信息
Weiss R E, Reddi A H
出版信息
J Cell Biol. 1981 Mar;88(3):630-6. doi: 10.1083/jcb.88.3.630.
Abstract
Fibronectin has been localized by indirect immunofluorescence during the various phases of endochondral bone formation in response to subcutaneously implanted demineralized bone matrix. Its histologic appearance has been correlated with results of biosynthetic experiments. (a) The implanted collagenous bone matrix was coated with fibronectin before and during mesenchymal cell proliferation. (b) During proliferation of mesenchymal precursor cells, the newly synthesized extracellular matrix exhibited a fibrillar network of fibronectin. (c) During cartilage differentiation, the fibronectin in the extracellular matrix was apparently masked by proteoglycans, as judged by hyaluronidase treatment. (d) Differentiating chondrocytes exhibited a uniform distribution of fibronectin. (e) Fibronectin was present in a cottony array around osteoblasts during osteogenesis. (f) The developing hematopoietic colonies revealed fibronectin associated with them. Therefore, it appears that fibronectin is ubiquitous throughout the development of endochondral bone and bone marrow.
摘要
在对皮下植入的脱矿骨基质的反应中，通过间接免疫荧光法对软骨内骨形成的各个阶段的纤连蛋白进行了定位。其组织学表现已与生物合成实验结果相关联。(a) 在间充质细胞增殖之前和期间，植入的胶原骨基质被纤连蛋白覆盖。(b) 在间充质前体细胞增殖期间，新合成的细胞外基质呈现出纤连蛋白的纤维状网络。(c) 在软骨分化期间，通过透明质酸酶处理判断，细胞外基质中的纤连蛋白显然被蛋白聚糖掩盖。(d) 分化中的软骨细胞呈现出纤连蛋白的均匀分布。(e) 在骨形成过程中，成骨细胞周围存在棉絮状排列的纤连蛋白。(f) 发育中的造血集落显示与其相关的纤连蛋白。因此，似乎纤连蛋白在软骨内骨和骨髓的整个发育过程中无处不在。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Changes in the patterns of collagens and fibronectin during limb-bud chondrogenesis.肢体芽软骨形成过程中胶原蛋白和纤连蛋白模式的变化。
J Embryol Exp Morphol. 1980 Jun;57:51-60.
3
Enzyme-linked immunoassay (ELISA) for connective tissue components.用于结缔组织成分的酶联免疫吸附测定（ELISA）。
Anal Biochem. 1980 May 1;104(1):205-14. doi: 10.1016/0003-2697(80)90300-0.
4
Synthesis and localization of fibronectin during collagenous matrix-mesenchymal cell interaction and differentiation of cartilage and bone in vivo.体内胶原基质与间充质细胞相互作用以及软骨和骨分化过程中纤连蛋白的合成与定位
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2074-8. doi: 10.1073/pnas.77.4.2074.
5
Fibronectin: a review of its structure and biological activity.纤连蛋白：其结构与生物活性综述
Mol Cell Biochem. 1980 Feb 8;29(2):103-28. doi: 10.1007/BF00220304.
6
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
7
Isolation of a collagen-dependent cell attachment factor.一种胶原依赖性细胞黏附因子的分离
Nature. 1974 Jul 19;250(463):248-51. doi: 10.1038/250248a0.
8
Biochemical sequences in the transformation of normal fibroblasts in adolescent rats.青春期大鼠正常成纤维细胞转化中的生化序列。
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9
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.通过荧光自显影对聚丙烯酰胺凝胶中的³H和¹⁴C进行定量胶片检测。
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