成年犬胫骨软骨来源的琼脂糖培养软骨细胞和分离软骨粒中VI型胶原蛋白与纤连蛋白的结构共定位
Structural colocalisation of type VI collagen and fibronectin in agarose cultured chondrocytes and isolated chondrons extracted from adult canine tibial cartilage.
作者信息
Chang J, Nakajima H, Poole C A
机构信息
Department of Anatomy, Faculty of Medicine and Health Sciences, University of Auckland, New Zealand.
出版信息
J Anat. 1997 May;190 ( Pt 4)(Pt 4):523-32. doi: 10.1046/j.1469-7580.1997.19040523.x.
Abstract
Cell-matrix and matrix-matrix interactions are of critical importance in regulating the development, maintenance and repair of articular cartilage. In this study, we examined the structural colocalisation of type VI collagen and fibronectin in isolated chondrons and long-term agarose cultured chondrocytes extracted from normal adult canine articular cartilage. Using double labelling immunohistochemistry in conjunction with dual channel confocal microscopy and digital image processing we demonstrate that type VI collagen and fibronectin are distributed in a similar staining pattern and are colocalised at the surface of cultured chondrocytes and isolated chondrons. The results suggest that type VI collagen and fibronectin may play a role in both cell-matrix adhesion and matrix-matrix cohesion in the pericellular microenvironment surrounding articular cartilage chondrocytes.
摘要
细胞与基质以及基质与基质之间的相互作用对于调节关节软骨的发育、维持和修复至关重要。在本研究中，我们检测了从正常成年犬关节软骨中提取的分离软骨细胞团和长期琼脂糖培养的软骨细胞中VI型胶原蛋白和纤连蛋白的结构共定位情况。通过结合双通道共聚焦显微镜和数字图像处理的双重标记免疫组织化学方法，我们证明VI型胶原蛋白和纤连蛋白以相似的染色模式分布，并在培养的软骨细胞和分离的软骨细胞团表面共定位。结果表明，VI型胶原蛋白和纤连蛋白可能在关节软骨软骨细胞周围的细胞外微环境中的细胞与基质黏附以及基质与基质黏附中发挥作用。
相似文献
1
Structural colocalisation of type VI collagen and fibronectin in agarose cultured chondrocytes and isolated chondrons extracted from adult canine tibial cartilage.成年犬胫骨软骨来源的琼脂糖培养软骨细胞和分离软骨粒中VI型胶原蛋白与纤连蛋白的结构共定位
J Anat. 1997 May;190 ( Pt 4)(Pt 4):523-32. doi: 10.1046/j.1469-7580.1997.19040523.x.
2
Chondrons from articular cartilage. V. Immunohistochemical evaluation of type VI collagen organisation in isolated chondrons by light, confocal and electron microscopy.来自关节软骨的软骨粒。V. 通过光学显微镜、共聚焦显微镜和电子显微镜对分离软骨粒中VI型胶原组织进行免疫组织化学评估。
J Cell Sci. 1992 Dec;103 ( Pt 4):1101-10. doi: 10.1242/jcs.103.4.1101.
3
In vitro culture of enzymatically isolated chondrons: a possible model for the initiation of osteoarthritis.酶解分离软骨颗粒的体外培养：骨关节炎起始的一种可能模型。
J Anat. 2006 Dec;209(6):793-806. doi: 10.1111/j.1469-7580.2006.00651.x.
4
Distribution of type VI collagen in chondrocyte microenvironment: study of chondrons isolated from human normal and degenerative articular cartilage and cultured chondrocytes.VI型胶原蛋白在软骨细胞微环境中的分布：对从人正常和退变关节软骨分离的软骨粒及培养软骨细胞的研究
J Orthop Sci. 2004;9(1):29-36. doi: 10.1007/s00776-003-0737-4.
5
Chondrons from articular cartilage: I. Immunolocalization of type VI collagen in the pericellular capsule of isolated canine tibial chondrons.来自关节软骨的软骨粒：I. 犬胫骨软骨粒细胞周围囊内VI型胶原蛋白的免疫定位
J Cell Sci. 1988 Aug;90 ( Pt 4):635-43. doi: 10.1242/jcs.90.4.635.
6
Chondrocyte deformation within mechanically and enzymatically extracted chondrons compressed in agarose.在琼脂糖中压缩的机械和酶法提取的软骨粒内的软骨细胞变形。
Biochim Biophys Acta. 2001 May 3;1526(2):141-6. doi: 10.1016/s0304-4165(01)00118-0.
7
Co-localization of insulin-like growth factor binding protein 3 and fibronectin in human articular cartilage.胰岛素样生长因子结合蛋白3与纤连蛋白在人关节软骨中的共定位
Osteoarthritis Cartilage. 2002 Jul;10(7):556-63. doi: 10.1053/joca.2002.0791.
8
Zonal variations in the three-dimensional morphology of the chondron measured in situ using confocal microscopy.使用共聚焦显微镜原位测量的软骨粒三维形态的区域变化。
Osteoarthritis Cartilage. 2006 Sep;14(9):889-97. doi: 10.1016/j.joca.2006.02.017. Epub 2006 Apr 19.
9
Chondrons from articular cartilage. (IV). Immunolocalization of proteoglycan epitopes in isolated canine tibial chondrons.来自关节软骨的软骨粒。（IV）。分离的犬胫骨软骨粒中蛋白聚糖表位的免疫定位。
J Histochem Cytochem. 1991 Sep;39(9):1175-87. doi: 10.1177/39.9.1717545.
10
Immunolocalization of type IX collagen in normal and spontaneously osteoarthritic canine tibial cartilage and isolated chondrons.IX型胶原蛋白在正常和自发性骨关节炎犬胫骨软骨及分离软骨细胞中的免疫定位
Osteoarthritis Cartilage. 1997 May;5(3):191-204. doi: 10.1016/s1063-4584(97)80014-3.
引用本文的文献
1
Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage.免疫荧光引导原子力显微镜测量猪关节软骨细胞外基质的微机械性能。
J R Soc Interface. 2012 Nov 7;9(76):2997-3007. doi: 10.1098/rsif.2012.0314. Epub 2012 Jun 6.
2
A biomechanical role for perlecan in the pericellular matrix of articular cartilage.蛋白聚糖在关节软骨细胞周基质中的生物力学作用。
Matrix Biol. 2012 Jul;31(6):320-7. doi: 10.1016/j.matbio.2012.05.002. Epub 2012 May 30.
3
Altered trabecular bone structure and delayed cartilage degeneration in the knees of collagen VI null mice.胶原 VI 基因敲除小鼠膝关节中小梁骨结构的改变和软骨退变的延迟。
PLoS One. 2012;7(3):e33397. doi: 10.1371/journal.pone.0033397. Epub 2012 Mar 20.
4
MMP mediated degradation of type VI collagen is highly associated with liver fibrosis--identification and validation of a novel biochemical marker assay.MMP 介导的 VI 型胶原降解与肝纤维化高度相关——新型生化标志物检测方法的鉴定和验证。
PLoS One. 2011;6(9):e24753. doi: 10.1371/journal.pone.0024753. Epub 2011 Sep 14.
5
Type II and VI collagen in nasal and articular cartilage and the effect of IL-1alpha on the distribution of these collagens.II 型和 VI 型胶原蛋白在鼻软骨和关节软骨中的分布及白介素-1α对其分布的影响。
J Mol Histol. 2010 Feb;41(1):9-17. doi: 10.1007/s10735-010-9257-7. Epub 2010 Mar 6.
6
Developmental and osteoarthritic changes in Col6a1-knockout mice: biomechanics of type VI collagen in the cartilage pericellular matrix.Col6a1基因敲除小鼠的发育和骨关节炎变化：软骨细胞周围基质中VI型胶原蛋白的生物力学
Arthritis Rheum. 2009 Mar;60(3):771-9. doi: 10.1002/art.24293.
7
Increased stromal extracellular matrix synthesis and assembly by insulin activated bovine keratocytes cultured under agarose.胰岛素激活的牛角膜细胞在琼脂糖下培养时，基质细胞外基质的合成和组装增加。
Exp Eye Res. 2008 Dec;87(6):604-11. doi: 10.1016/j.exer.2008.09.010. Epub 2008 Oct 7.
8
Requirements for sulfate transport and the diastrophic dysplasia sulfate transporter in fibronectin matrix assembly.纤连蛋白基质组装中硫酸盐转运及畸形性发育不良硫酸盐转运体的要求。
J Cell Biol. 2007 Dec 3;179(5):999-1009. doi: 10.1083/jcb.200707150.
9
In vitro culture of enzymatically isolated chondrons: a possible model for the initiation of osteoarthritis.酶解分离软骨颗粒的体外培养：骨关节炎起始的一种可能模型。
J Anat. 2006 Dec;209(6):793-806. doi: 10.1111/j.1469-7580.2006.00651.x.
10
The distribution of type VI collagen in the developing tissues of the bovine femoral head.VI型胶原蛋白在牛股骨头发育组织中的分布。
Histochem J. 1999 Sep;31(9):623-32. doi: 10.1023/a:1003811310619.
本文引用的文献
1
Detection of viable and non-viable cells in connective tissue explants using the fixable fluoroprobes 5-chloromethylfluorescein diacetate and ethidium homodimer-1.使用可固定荧光探针5-氯甲基荧光素二乙酸酯和碘化丙啶同源二聚体-1检测结缔组织外植体中的活细胞和死细胞。
Connect Tissue Res. 1996;33(4):233-41. doi: 10.3109/03008209609028880.
2
Increased cell diameter precedes chondrocyte terminal differentiation, whereas cell-matrix attachment complex proteins appear constant.细胞直径增加先于软骨细胞终末分化，而细胞-基质附着复合体蛋白似乎保持不变。
Anat Rec. 1996 Mar;244(3):284-96. doi: 10.1002/(SICI)1097-0185(199603)244:3<284::AID-AR2>3.0.CO;2-Z.
3
4
Fibronectin, cartilage, and osteoarthritis.纤连蛋白、软骨与骨关节炎
Semin Arthritis Rheum. 1993 Apr;22(5):307-18. doi: 10.1016/s0049-0172(05)80010-1.
5
Extracellular matrix regulates expression of the TGF-beta 1 gene.细胞外基质调节转化生长因子β1基因的表达。
J Cell Biol. 1993 Jan;120(1):253-60. doi: 10.1083/jcb.120.1.253.
6
Integrins in ageing cartilage tissue in vitro.体外老化软骨组织中的整合素
Histol Histopathol. 1993 Oct;8(4):715-23.
7
The dynamic structure of the pericellular matrix on living cells.活细胞周围细胞外基质的动态结构。
J Cell Biol. 1993 Dec;123(6 Pt 2):1899-907. doi: 10.1083/jcb.123.6.1899.
8
Chondrocyte heterogeneity: immunohistologically defined variation of integrin expression at different sites in human fetal knees.软骨细胞异质性：人胎儿膝关节不同部位整合素表达的免疫组织学定义变异
J Histochem Cytochem. 1995 Apr;43(4):447-57. doi: 10.1177/43.4.7897185.
9
Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.透明质酸受体导向的软骨细胞周围基质组装。
J Cell Biol. 1993 Feb;120(3):825-34. doi: 10.1083/jcb.120.3.825.
10
Type VI collagen but not type V collagen is present in cartilage.VI型胶原蛋白存在于软骨中，而V型胶原蛋白则不存在。
Coll Relat Res. 1984 Mar;4(2):165-8. doi: 10.1016/s0174-173x(84)80023-0.
Zotero 插件