成年犬胫骨软骨来源的琼脂糖培养软骨细胞和分离软骨粒中VI型胶原蛋白与纤连蛋白的结构共定位
Structural colocalisation of type VI collagen and fibronectin in agarose cultured chondrocytes and isolated chondrons extracted from adult canine tibial cartilage.
作者信息
Chang J, Nakajima H, Poole C A
机构信息
Department of Anatomy, Faculty of Medicine and Health Sciences, University of Auckland, New Zealand.
出版信息
J Anat. 1997 May;190 ( Pt 4)(Pt 4):523-32. doi: 10.1046/j.1469-7580.1997.19040523.x.
Abstract
Cell-matrix and matrix-matrix interactions are of critical importance in regulating the development, maintenance and repair of articular cartilage. In this study, we examined the structural colocalisation of type VI collagen and fibronectin in isolated chondrons and long-term agarose cultured chondrocytes extracted from normal adult canine articular cartilage. Using double labelling immunohistochemistry in conjunction with dual channel confocal microscopy and digital image processing we demonstrate that type VI collagen and fibronectin are distributed in a similar staining pattern and are colocalised at the surface of cultured chondrocytes and isolated chondrons. The results suggest that type VI collagen and fibronectin may play a role in both cell-matrix adhesion and matrix-matrix cohesion in the pericellular microenvironment surrounding articular cartilage chondrocytes.
摘要
细胞与基质以及基质与基质之间的相互作用对于调节关节软骨的发育、维持和修复至关重要。在本研究中，我们检测了从正常成年犬关节软骨中提取的分离软骨细胞团和长期琼脂糖培养的软骨细胞中VI型胶原蛋白和纤连蛋白的结构共定位情况。通过结合双通道共聚焦显微镜和数字图像处理的双重标记免疫组织化学方法，我们证明VI型胶原蛋白和纤连蛋白以相似的染色模式分布，并在培养的软骨细胞和分离的软骨细胞团表面共定位。结果表明，VI型胶原蛋白和纤连蛋白可能在关节软骨软骨细胞周围的细胞外微环境中的细胞与基质黏附以及基质与基质黏附中发挥作用。
相似文献
1
Structural colocalisation of type VI collagen and fibronectin in agarose cultured chondrocytes and isolated chondrons extracted from adult canine tibial cartilage.
J Anat. 1997 May;190 ( Pt 4)(Pt 4):523-32. doi: 10.1046/j.1469-7580.1997.19040523.x.
2
Chondrons from articular cartilage. V. Immunohistochemical evaluation of type VI collagen organisation in isolated chondrons by light, confocal and electron microscopy.
J Cell Sci. 1992 Dec;103 ( Pt 4):1101-10. doi: 10.1242/jcs.103.4.1101.
3
In vitro culture of enzymatically isolated chondrons: a possible model for the initiation of osteoarthritis.
J Anat. 2006 Dec;209(6):793-806. doi: 10.1111/j.1469-7580.2006.00651.x.
4
Distribution of type VI collagen in chondrocyte microenvironment: study of chondrons isolated from human normal and degenerative articular cartilage and cultured chondrocytes.
J Orthop Sci. 2004;9(1):29-36. doi: 10.1007/s00776-003-0737-4.
5
Chondrons from articular cartilage: I. Immunolocalization of type VI collagen in the pericellular capsule of isolated canine tibial chondrons.
J Cell Sci. 1988 Aug;90 ( Pt 4):635-43. doi: 10.1242/jcs.90.4.635.
6
Chondrocyte deformation within mechanically and enzymatically extracted chondrons compressed in agarose.
Biochim Biophys Acta. 2001 May 3;1526(2):141-6. doi: 10.1016/s0304-4165(01)00118-0.
7
Co-localization of insulin-like growth factor binding protein 3 and fibronectin in human articular cartilage.
Osteoarthritis Cartilage. 2002 Jul;10(7):556-63. doi: 10.1053/joca.2002.0791.
8
Zonal variations in the three-dimensional morphology of the chondron measured in situ using confocal microscopy.
Osteoarthritis Cartilage. 2006 Sep;14(9):889-97. doi: 10.1016/j.joca.2006.02.017. Epub 2006 Apr 19.
9
Chondrons from articular cartilage. (IV). Immunolocalization of proteoglycan epitopes in isolated canine tibial chondrons.
J Histochem Cytochem. 1991 Sep;39(9):1175-87. doi: 10.1177/39.9.1717545.
10
Immunolocalization of type IX collagen in normal and spontaneously osteoarthritic canine tibial cartilage and isolated chondrons.
Osteoarthritis Cartilage. 1997 May;5(3):191-204. doi: 10.1016/s1063-4584(97)80014-3.
引用本文的文献
1
Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage.
J R Soc Interface. 2012 Nov 7;9(76):2997-3007. doi: 10.1098/rsif.2012.0314. Epub 2012 Jun 6.
2
A biomechanical role for perlecan in the pericellular matrix of articular cartilage.
Matrix Biol. 2012 Jul;31(6):320-7. doi: 10.1016/j.matbio.2012.05.002. Epub 2012 May 30.
3
Altered trabecular bone structure and delayed cartilage degeneration in the knees of collagen VI null mice.
PLoS One. 2012;7(3):e33397. doi: 10.1371/journal.pone.0033397. Epub 2012 Mar 20.
4
MMP mediated degradation of type VI collagen is highly associated with liver fibrosis--identification and validation of a novel biochemical marker assay.
PLoS One. 2011;6(9):e24753. doi: 10.1371/journal.pone.0024753. Epub 2011 Sep 14.
5
Type II and VI collagen in nasal and articular cartilage and the effect of IL-1alpha on the distribution of these collagens.
J Mol Histol. 2010 Feb;41(1):9-17. doi: 10.1007/s10735-010-9257-7. Epub 2010 Mar 6.
6
Developmental and osteoarthritic changes in Col6a1-knockout mice: biomechanics of type VI collagen in the cartilage pericellular matrix.
Arthritis Rheum. 2009 Mar;60(3):771-9. doi: 10.1002/art.24293.
7
Increased stromal extracellular matrix synthesis and assembly by insulin activated bovine keratocytes cultured under agarose.
Exp Eye Res. 2008 Dec;87(6):604-11. doi: 10.1016/j.exer.2008.09.010. Epub 2008 Oct 7.
8
Requirements for sulfate transport and the diastrophic dysplasia sulfate transporter in fibronectin matrix assembly.
J Cell Biol. 2007 Dec 3;179(5):999-1009. doi: 10.1083/jcb.200707150.
9
In vitro culture of enzymatically isolated chondrons: a possible model for the initiation of osteoarthritis.
J Anat. 2006 Dec;209(6):793-806. doi: 10.1111/j.1469-7580.2006.00651.x.
10
The distribution of type VI collagen in the developing tissues of the bovine femoral head.
Histochem J. 1999 Sep;31(9):623-32. doi: 10.1023/a:1003811310619.
本文引用的文献
1
Detection of viable and non-viable cells in connective tissue explants using the fixable fluoroprobes 5-chloromethylfluorescein diacetate and ethidium homodimer-1.
Connect Tissue Res. 1996;33(4):233-41. doi: 10.3109/03008209609028880.
2
Increased cell diameter precedes chondrocyte terminal differentiation, whereas cell-matrix attachment complex proteins appear constant.
Anat Rec. 1996 Mar;244(3):284-96. doi: 10.1002/(SICI)1097-0185(199603)244:3<284::AID-AR2>3.0.CO;2-Z.
3
Fibronectin induces protease dependent focal matrix depletion and cell overlap in cultured rheumatoid synoviocytes.
J Rheumatol. 1995 May;22(5):817-28.
4
Fibronectin, cartilage, and osteoarthritis.
Semin Arthritis Rheum. 1993 Apr;22(5):307-18. doi: 10.1016/s0049-0172(05)80010-1.
5
Extracellular matrix regulates expression of the TGF-beta 1 gene.
J Cell Biol. 1993 Jan;120(1):253-60. doi: 10.1083/jcb.120.1.253.
6
Integrins in ageing cartilage tissue in vitro.
Histol Histopathol. 1993 Oct;8(4):715-23.
7
The dynamic structure of the pericellular matrix on living cells.
J Cell Biol. 1993 Dec;123(6 Pt 2):1899-907. doi: 10.1083/jcb.123.6.1899.
8
Chondrocyte heterogeneity: immunohistologically defined variation of integrin expression at different sites in human fetal knees.
J Histochem Cytochem. 1995 Apr;43(4):447-57. doi: 10.1177/43.4.7897185.
9
Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.
J Cell Biol. 1993 Feb;120(3):825-34. doi: 10.1083/jcb.120.3.825.
10
Type VI collagen but not type V collagen is present in cartilage.
Coll Relat Res. 1984 Mar;4(2):165-8. doi: 10.1016/s0174-173x(84)80023-0.
Zotero 插件