Virts E L, Gelvin S B
J Bacteriol. 1985 Jun;162(3):1030-8. doi: 10.1128/jb.162.3.1030-1038.1985.
Petunia protoplasts were infected with the virulent Agrobacterium tumefaciens strain A348 or the avirulent strain A136 (lacking a Ti plasmid). The infection process was stopped at various time intervals up to 24 h after inoculation, and the DNA from the plant cells was isolated. Southern blot analysis indicated that the DNA isolated from infected Petunia cells was not detectably contaminated by bacterial DNA from lysed Agrobacterium cells. Analysis of the DNA from the virulent infections suggested that the transferred DNA (T-DNA) may be transferred to the plant cell rapidly (within 2 to 6 h) after the bacteria bind to the cell wall and that the T-DNA may exist in a rearranged state which is stable over the time period investigated. Dot blot analysis indicated that regions far outside the T-DNA may be transferred to the plant cell. Most of the DNA transferred to the plant cell during the initial hours of infection is rapidly degraded.
用强毒根癌土壤杆菌菌株A348或无毒菌株A136（缺乏Ti质粒）感染矮牵牛原生质体。在接种后长达24小时的不同时间间隔停止感染过程，并分离植物细胞的DNA。Southern印迹分析表明，从感染的矮牵牛细胞中分离的DNA未被来自裂解的土壤杆菌细胞的细菌DNA明显污染。对强毒感染的DNA分析表明，转移DNA（T-DNA）可能在细菌与细胞壁结合后迅速（2至6小时内）转移到植物细胞中，并且T-DNA可能以重排状态存在，在所研究的时间段内是稳定的。斑点印迹分析表明，T-DNA之外的远区域可能转移到植物细胞中。在感染最初几小时内转移到植物细胞中的大部分DNA迅速降解。
