Yang Chunlei, Xu Guiju, Hou Chenghao, Zhang Hongwei
Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Sci Rep. 2025 Feb 20;15(1):6261. doi: 10.1038/s41598-025-89327-z.
Heavy metal ions pollution in environmental waters has an increasing impact on human health. As two common metal ions, copper ions (Cu) and ferric ions (Fe) widely exist in nature and play a vital role in life process. Therefore, it is significant to design sensitive and simple detection approaches for Cu and Fe. In our work, the ratiometric fluorescence analysis method (denoted as N-CDs/OPD) was established for Cu and Fe detection. The N-CDs exhibited a Cu and Fe fluorescence quenching response properties. The o-phenylenediamine (OPD) may be oxidized to 2,3-diaminophenazine (DAP) by Cu and Fe. With addition of Cu or Fe, the fluorescence of N-CDs (436 nm) was quenched and a new peak at 556 nm (DAP) appeared, which realized fluorescent ratiometric detection of Cu and Fe. The Cu concentration shows a good linear correlation versus fluorescence ratio (F/F) in the range of 10 to 30 µM (R = 0.9981) with detection limit (LOD) of 0.86 µM. In addition, a good linear relationship between fluorescence ratio (F/F) and Fe concentration in the range of 20 to 80 µM (R = 0.9880) with LOD of 7.12 µM. This nanoprobe realizes the detection of authentic samples successfully, which is expected to serve as a testing kit for analysis in water samples.
环境水体中的重金属离子污染对人类健康的影响日益增大。作为两种常见的金属离子，铜离子（Cu）和铁离子（Fe）广泛存在于自然界中，并且在生命过程中发挥着至关重要的作用。因此，设计灵敏且简便的铜离子和铁离子检测方法具有重要意义。在我们的工作中，建立了用于检测铜离子和铁离子的比率荧光分析法（记为N-CDs/OPD）。N-CDs表现出对铜离子和铁离子的荧光猝灭响应特性。邻苯二胺（OPD）可能会被铜离子和铁离子氧化为2,3-二氨基吩嗪（DAP）。加入铜离子或铁离子后，N-CDs（436 nm）的荧光被猝灭，并且在556 nm处出现一个新的峰（DAP），从而实现了对铜离子和铁离子的荧光比率检测。在10至30 μM范围内，铜离子浓度与荧光比率（F/F）呈现良好的线性相关性（R = 0.9981），检测限（LOD）为0.86 μM。此外，在20至80 μM范围内，荧光比率（F/F）与铁离子浓度之间具有良好的线性关系（R = 0.9880），LOD为7.12 μM。该纳米探针成功实现了对实际样品的检测，有望作为一种用于水样分析的检测试剂盒。
