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大豆过氧化物酶在分级沸石-有序介孔碳纳米复合材料上的固定化及其活性。

Immobilization of soybean peroxidase enzyme on hierarchical zeolite-ordered mesoporous carbon nanocomposite and its activity.

作者信息

Azhagapillai Prabhu, Gopalsamy Karthikeyan, Othman Israa, Alhatti Nada I, Haija Mohammad Abu, Ashraf Syed Salman

机构信息

Department of Chemistry, Khalifa University P. O. Box 127788 Abu Dhabi United Arab Emirates

Department of Biological Sciences, Khalifa University P. O. Box 127788 Abu Dhabi United Arab Emirates

出版信息

RSC Adv. 2025 Feb 20;15(8):5781-5794. doi: 10.1039/d4ra07503j. eCollection 2025 Feb 19.

DOI:10.1039/d4ra07503j
PMID:39980993
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11840808/
Abstract

Immobilization of enzymes on inorganic supports such as silica and carbon materials is an effective approach for chemical surface modification. In this work, hierarchical zeolite (HZ-SAPO's) materials were fabricated by a modified method, and mesoporous carbon (CMK-3) was synthesized using the SBA-15 mesoporous silica as a template. A variety of biocatalysts was prepared using HZ-SAPO with CMK to furnish the nanocomposite biocatalyst. The functionalization of amine group with APTES was done which was further immobilized by Soybean Peroxidase (SBP) enzyme. The material was subjected to a comprehensive characterization process utilizing numerous systematic methods, including X-ray diffraction, N adsorption-desorption isotherms, Raman spectroscopy, scanning electron microscopy, high-resolution transmittance electron microscopy, and attenuated total reflectance Fourier transform infrared spectroscopy. The pH effect on the immobilized enzyme was examined and compared to that of SBP. Further, the assessment of repeated usability of immobilized SBP with successive cycles was carried out.

摘要

将酶固定在无机载体（如二氧化硅和碳材料）上是一种有效的化学表面改性方法。在这项工作中，采用改进的方法制备了分级沸石（HZ-SAPO's）材料，并以SBA-15介孔二氧化硅为模板合成了介孔碳（CMK-3）。使用HZ-SAPO和CMK制备了多种生物催化剂，以提供纳米复合生物催化剂。用APTES对胺基进行功能化，然后通过大豆过氧化物酶（SBP）进一步固定。利用多种系统方法对该材料进行了全面表征，包括X射线衍射、N吸附-脱附等温线、拉曼光谱、扫描电子显微镜、高分辨率透射电子显微镜和衰减全反射傅里叶变换红外光谱。研究了pH对固定化酶的影响，并与SBP的影响进行了比较。此外，还对固定化SBP连续循环的重复使用性进行了评估。

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本文引用的文献

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