Strong V F, Sokol R J, Rodgers S A, Hewitt S
J Clin Pathol. 1985 May;38(5):585-7. doi: 10.1136/jcp.38.5.585.
Erythrocyte adenine nucleotide concentrations were measured in 154 patients with erythrocyte autoantibodies and 811 normal subjects using a luciferin-luciferase bioluminescent assay. The patients were initially divided into haemolysing and non-haemolysing groups. Red cell adenosine triphosphate (ATP) concentrations were significantly raised in the 96 patients with active haemolysis compared with the normal subjects and with the 58 patients in the non-haemolysing group. Although the patients categorised as non-haemolysing had higher ATP values than the normal subjects, this was because several patients had the anaemia of chronic disorders, which is associated with a shortened red cell life. Measurement of red cell ATP was a useful indicator of mean erythrocyte age and a raised value in patients with erythrocyte autoantibodies reflected active haemolysis.
采用荧光素 - 荧光素酶生物发光测定法，对154例患有红细胞自身抗体的患者和811名正常受试者的红细胞腺嘌呤核苷酸浓度进行了测量。患者最初被分为溶血组和非溶血组。与正常受试者以及非溶血组的58例患者相比，96例有活动性溶血的患者红细胞三磷酸腺苷（ATP）浓度显著升高。虽然分类为非溶血的患者ATP值高于正常受试者，但这是因为部分患者患有慢性疾病性贫血，这与红细胞寿命缩短有关。红细胞ATP的测量是平均红细胞年龄的有用指标，红细胞自身抗体患者中升高的值反映了活动性溶血。
