Bisicchia R, Zunin A, Donisi C
Boll Ist Sieroter Milan. 1979 Nov 30;58(5):382-90.
The Authors illustrate two modifications of the indirect method for identifying Salmonellae on faeces using fluorescent antibodies. They compare a Commercial polyvalent serum with serum containing only the fundamental antigens of groups B, C and D of Kauffmann-White with the aim of reducing the phenomenon of aspecificity. In order to reduce the time required for this test they substitute the enrichment of the specimen in broth by filtering, first the specimen of faeces and then, successively, centrifuging the filtrate. These two modifications have been demonstrated satisfactorily when the test is carried out in children with active gastroenteritis.
作者阐述了两种使用荧光抗体在粪便中鉴定沙门氏菌的间接方法的改进。他们将一种商业多价血清与仅含有考夫曼-怀特B、C和D组基本抗原的血清进行比较，目的是减少非特异性现象。为了减少该检测所需的时间，他们通过过滤替代在肉汤中对标本进行增菌，先过滤粪便标本，然后依次对滤液进行离心。当在患有急性肠胃炎的儿童中进行该检测时，这两种改进已得到令人满意的验证。
