Boschiero Clarissa, Beshah Ethiopia, Bakshi Mariam, Thompson Peter, Zhu Xiaoping, Liu George E, Tuo Wenbin
Animal Parasitic Diseases Laboratory, BARC, Agricultural Research Service, USDA, Beltsville, Maryland, 20705, USA.
Department of Veterinary Medicine, University of Maryland, College Park, Maryland, 20742, USA.
Sci Data. 2025 Feb 21;12(1):315. doi: 10.1038/s41597-025-04640-6.
In this study, we infected Holstein calves with Ostertagia ostertagi stage 3 larvae (L3) and determined gene expression profiles of abomasal fundic and pyloric mucosa by RNA sequencing (RNA-seq) at 3-5, 7-9, 10, and 21 days post-infection (dpi), which represent late L3 and early L4 (between 3-5 dpi), mid to late L4 (between 7-9 and 10 dpi) and adult stages (21 dpi) of the parasitic stage. Bioinformatics analyses were performed to profile the transcriptomic changes over time as well as between cattle abomasal tissues. The results will help understand the gastric responses of the host, especially the immune responses, at different phases of early nematode infection. Such an undertaking is crucial for an in-depth comprehension of host responses, where immunologic reagents for cattle are highly limited. The RNA-seq datasets generated in this study provide a vital data resource, allowing for future comparative analyses to similar data resources or using more advanced analytical technologies in mechanisms of host-parasite interactions.
在本研究中，我们用奥斯特他线虫三期幼虫（L3）感染荷斯坦犊牛，并在感染后3至5天、7至9天、10天和21天通过RNA测序（RNA-seq）确定皱胃底部和幽门黏膜的基因表达谱，这些时间点分别代表寄生虫阶段的晚期L3和早期L4（感染后3至5天之间）、中期至晚期L4（感染后7至9天和10天之间）以及成虫阶段（感染后21天）。进行了生物信息学分析，以描绘随时间以及牛皱胃组织之间的转录组变化。这些结果将有助于了解宿主在早期线虫感染不同阶段的胃部反应，尤其是免疫反应。鉴于用于牛的免疫试剂非常有限，这样的研究对于深入理解宿主反应至关重要。本研究中生成的RNA-seq数据集提供了重要的数据资源，允许未来与类似数据资源进行比较分析，或在宿主-寄生虫相互作用机制中使用更先进的分析技术。
