Qiao Qian, Shen Bingxue, Lin Ke, Zhu Dongzi, Hong Po, Zhang Lisi, Sun Jiazheng, Sun Shan, Gao Yun, Zhang Shizhong, Wang Jiawei, Liu Qingzhong
Shandong Key Laboratory of Fruit Biotechnology Breeding, Shandong Institute of Pomology, Taian, 271000, Shandong, China.
State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018, Shandong, China.
Sci Rep. 2025 Feb 21;15(1):6311. doi: 10.1038/s41598-025-85821-6.
In the cultivation and production of sweet cherry, the cost of picking fruit is high due to inconsistency in the maturation period, which has affected the development of the cherry industry. In this study, the effects of exogenous abscisic acid (ABA) on the sweet cherry variety 'Luying 3' fruit quality and maturation stage were observed and recorded, and the physiological and molecular mechanisms were explored to systematically analyze the effects of ABA on sweet cherry fruit ripening to promote the development of the cherry industry. Exogenous ABA (400 mg L) enhanced the color of 'Luying 3' fruit in the developing stage but had no significant effect on the fruit weight, soluble solid content, titratable acid content, and sugar-acid ratio in the mature stage. The application of ABA significantly promoted the secretion of endogenous ABA, gibberellin (GA) and salicylic acid (SA). A total of 766 differentially expressed genes (DEGs) were obtained between the treatment group and the control group at 47 and 54 d after flowering. The DEGs were significantly enriched in plant hormone signal transduction pathway, MAPK plant signal transduction pathway and glycolysis pathway. Six genes related to the synthesis of endogenous hormones were screened, of which five were upregulated and one was downregulated. Four DEGs related to the sweet cherry fruit metabolic rate were upregulated by ABA, which positively regulated fruit ripening. Eight differentially expressed AP2/ERF transcription factors were identified, of which 5 were upregulated and 3 were downregulated. This study provides a theoretical foundation for the application of ABA in promoting the consistency of cherry fruit maturity.
在甜樱桃的栽培生产中，由于成熟期不一致，果实采摘成本较高，这影响了樱桃产业的发展。本研究观察并记录了外源脱落酸（ABA）对甜樱桃品种‘鲁樱3号’果实品质和成熟阶段的影响，探讨了其生理和分子机制，以系统分析ABA对甜樱桃果实成熟的影响，促进樱桃产业发展。外源ABA（400 mg·L）在发育阶段增强了‘鲁樱3号’果实的色泽，但对成熟阶段的果实重量、可溶性固形物含量、可滴定酸含量和糖酸比无显著影响。ABA的施用显著促进了内源ABA、赤霉素（GA）和水杨酸（SA）的分泌。在开花后47和54 d，处理组和对照组之间共获得766个差异表达基因（DEG）。这些DEG在植物激素信号转导途径、MAPK植物信号转导途径和糖酵解途径中显著富集。筛选出6个与内源激素合成相关的基因，其中5个上调，1个下调。4个与甜樱桃果实代谢率相关的DEG被ABA上调，对果实成熟起正向调控作用。鉴定出8个差异表达的AP2/ERF转录因子，其中5个上调，3个下调。本研究为ABA在促进樱桃果实成熟一致性方面的应用提供了理论基础。
