Ohta T
J Exp Zool. 1985 May;234(2):273-81. doi: 10.1002/jez.1402340213.
Unfertilized eggs of the rose bitterling (Rhodeus ocellatus ocellatus) were squeezed out of females that had an elongated ovipositor and were dechorionated mechanically with fine forceps in physiological saline. The dechorionated eggs were transferred into fresh water then inseminated at once by spermatozoa of the same species. A large number of spermatozoa was found on the surface of eggs that had not yet had cortical reaction following insemination. The surface of the naked eggs responded by formation of many small cytoplasmic protrusions (viz., fertilization cones) at sperm attachment sites. The formed fertilization cones were rosettelike structures formed by the aggregation of some bleblike swellings devoid of microvilli and microplicae. About 10 min after insemination, the fertilization cones retracted, but marks of their presence characterized by less microvilli and microplicae remained in the eggs 15 min after insemination. Many spermatozoa penetrated into the cytoplasm of each naked egg. The sperm nuclear envelope disappeared by means of vesiculation resulting from fusion of the inner and outer membranes. The sperm nucleus decondensed and developed into a larger male pronucleus. Smooth-surfaced vesicles surrounded the decondensing sperm nucleus and formed the new male pronuclear envelope. Sperm mitochondria and flagella were found in the egg 15 min after insemination. The response of the egg surface to sperm entry and pronucleus formation are discussed.
将具有延长产卵器的玫瑰无须鲃（Rhodeus ocellatus ocellatus）雌鱼体内的未受精卵挤出，在生理盐水中用精细镊子机械去除卵膜。将去膜后的卵转移到淡水中，然后立即用同种精子进行授精。在授精后尚未发生皮层反应的卵表面发现大量精子。裸露卵的表面在精子附着部位形成许多小的细胞质突起（即受精锥）作为反应。形成的受精锥是由一些没有微绒毛和微褶的泡状肿胀聚集而成的玫瑰花状结构。授精后约10分钟，受精锥缩回，但授精后15分钟，卵中仍保留着以微绒毛和微褶较少为特征的它们存在的痕迹。许多精子穿透到每个裸露卵的细胞质中。精子核膜通过内膜和外膜融合产生的小泡化而消失。精子核解聚并发育成一个更大的雄原核。表面光滑的小泡围绕着解聚的精子核并形成新的雄原核膜。授精后15分钟在卵中发现精子线粒体和鞭毛。本文讨论了卵表面对精子进入和原核形成的反应。
