Aiello Gilda, Cropotova Janna, Kvangarsnes Kristine, d'Adduzio Lorenza, Fanzaga Melissa, Bollati Carlotta, Boschin Giovanna, Roda Gabriella, Lammi Carmen
Department of Human Science and Quality of Life Promotion, Telematic University San Raffaele, Rome, Italy.
Department of Biological Sciences Ålesund, Norwegian University of Science and Technology (NTNU), Ålesund, Norway.
NPJ Sci Food. 2025 Feb 22;9(1):25. doi: 10.1038/s41538-025-00388-w.
This study demonstrates the effectiveness of ultrasonication, as a pre-treatment technology, coupled to enzymatic hydrolysis of herring side streams, yielding multifunctional peptide mixtures with antioxidant, hypotensive (ACE inhibitory activity), and hypoglycemic (DPP-IV inhibitory and GLP-1 enhancer activity) properties. The ultrasound pre-treatment modulates the biological activity of the hydrolysates, enhancing certain bioactive properties (antioxidant, ACE inhibitory, and GLP-1 enhancer activities, respectively) while reducing others (DPP-IV inhibitory activity). The study also highlights the importance of simulating gastrointestinal digestion and using Caco-2 cells to assess the bioaccessibility, intestinal bioavailability, and metabolic resistance of herring peptides. These findings support the use of ultrasonication and enzymatic hydrolysis in obtaining multifunctional bioactive peptide mixture for the prevention of metabolic syndrome. Results clearly suggest that this approach represent sustainable solutions in food science and technology, since it allowed us to obtain a bioactive mixture of peptides starting from fish by-products pre-treated with green methodologies.
本研究证明了超声处理作为一种预处理技术与鲱鱼副产物酶解相结合的有效性，可产生具有抗氧化、降血压（ACE抑制活性）和降血糖（DPP-IV抑制和GLP-1增强活性）特性的多功能肽混合物。超声预处理调节了水解产物的生物活性，增强了某些生物活性特性（分别为抗氧化、ACE抑制和GLP-1增强活性），同时降低了其他特性（DPP-IV抑制活性）。该研究还强调了模拟胃肠道消化以及使用Caco-2细胞评估鲱鱼肽的生物可及性、肠道生物利用度和代谢抗性的重要性。这些发现支持使用超声处理和酶解来获得用于预防代谢综合征的多功能生物活性肽混合物。结果清楚地表明，这种方法代表了食品科学与技术中的可持续解决方案，因为它使我们能够从采用绿色方法预处理的鱼副产物中获得肽的生物活性混合物。
