Huang Tristan Yusho, Yang John Jackson
Arizona College of Osteopathic Medicine, Midwestern University, Arizona, 85308, USA.
Department of Medical Biochemistry, Universitas Kristen Indonesia, Jakarta, 13630, Indonesia.
Microb Cell Fact. 2025 Feb 22;24(1):46. doi: 10.1186/s12934-025-02673-5.
Leuconostoc mesenteroides (L. mesenteroides) has known as an electrogenic probiotic bacterium. However, metabolites related to electro-fermentation in ferments of L. mesenteroides are not unveiled.
Electrogenic L. mesenteroides fermentatively metabolized bovine milk to dense ferments with homogeneous particle-size distribution. A non-targeted metabolomics approach was performed on non-fermented and L. mesenteroides-fermented milk. A total of 917 metabolites were identified and quantified by ultra-high performance liquid chromatography (UHPLC)-tandem mass spectrometry (MS-MS). Thirteen prokaryotic metabolic pathways associated with differentially expressed metabolites (DEMs) were revealed through Koto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Anthranilic acid (AA) and 3-hydroxyanthranilin acid (3-HAA), potentially as electron donors, and quinolinic acid, an electron donor precursor, in the tryptophan kynurenine pathway were significantly increased in the fermented milk. Histidine, arginine, and riboflavin involved in bacterial survival or bioelectricity production were elevated after fermentation.
Results indicate that electrogenic L. mesenteroides can mediate electro-fermentation to transform milk to a new nutritional source which is rich in electron donors reportedly acting as antioxidants.
肠膜明串珠菌（L. mesenteroides）被认为是一种产电益生菌。然而，肠膜明串珠菌发酵产物中与电发酵相关的代谢产物尚未被揭示。
产电的肠膜明串珠菌通过发酵将牛乳代谢为具有均匀粒度分布的浓稠发酵产物。对未发酵和经肠膜明串珠菌发酵的牛奶进行了非靶向代谢组学分析。通过超高效液相色谱（UHPLC）-串联质谱（MS-MS）共鉴定和定量了917种代谢产物。通过京都基因与基因组百科全书（KEGG）富集分析揭示了13条与差异表达代谢产物（DEM）相关的原核生物代谢途径。色氨酸犬尿氨酸途径中的邻氨基苯甲酸（AA）和3-羟基邻氨基苯甲酸（3-HAA）可能作为电子供体，以及电子供体前体喹啉酸在发酵乳中显著增加。参与细菌存活或生物电产生的组氨酸、精氨酸和核黄素在发酵后升高。
结果表明，产电的肠膜明串珠菌可介导电发酵，将牛奶转化为一种新的营养源，据报道这种营养源富含作为抗氧化剂的电子供体。
