Gong Xiong, Ding Zufa, Jiang Haifeng, Wang Cheng, Qian Yuting, Chen Wenjun, Yu Dan, Liu Yang, Zheng Lingling, Yang Liandong, Geng Longwu
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China.
Key Laboratory of Cold Water Fish Germplasm Resources and Multiplication and Cultivation of Heilongjiang Province, Harbin, 150070, China.
Sci Data. 2025 Feb 22;12(1):321. doi: 10.1038/s41597-025-04661-1.
Pseudaspius leptocephalus is an economically and ecologically important fish species endemic to the Heilongjiang River Basin. Sequencing of the complete genome of P. leptocephalus could provide new insights with implications for the breeding and conservation of this species. The aim of this study was to obtain the chromosome-level genome of P. leptocephalus using PacBio and Hi-C sequencing. PacBio sequencing yielded an assembly of 889.21 Mb, with a contig N50 size of 10.9 Mb. Hi-C sequencing data were used for chromosome mapping and ultimately yielded 25 chromosome sequences. The success rate of chromosome mapping was 95.0%, with a BUSCO evaluation integrity score of 97.3%. A total of 25,531 protein-coding genes were predicted and 24,787 genes were functionally annotated. The BUSCO evaluation integrity score for the predicted protein-coding genes was 93.7%. Repeat sequences accounted for 39.5% of the total length of the genome. This work could aid the artificial breeding of P. leptocephalus, promote the conservation of biological resources, and provide a basis for future genomic and evolutionary studies of this species.
细头拟赤梢鱼是黑龙江流域特有的一种具有经济和生态重要性的鱼类。细头拟赤梢鱼全基因组测序可为该物种的育种和保护提供新的见解。本研究的目的是利用PacBio和Hi-C测序获得细头拟赤梢鱼的染色体水平基因组。PacBio测序产生了一个889.21 Mb的组装体，重叠群N50大小为10.9 Mb。Hi-C测序数据用于染色体定位，最终产生了25条染色体序列。染色体定位成功率为95.0%，BUSCO评估完整性得分97.3%。共预测出25,531个蛋白质编码基因，其中24,787个基因得到功能注释。预测的蛋白质编码基因的BUSCO评估完整性得分为93.7%。重复序列占基因组总长度的39.5%。这项工作有助于细头拟赤梢鱼的人工养殖，促进生物资源的保护，并为该物种未来的基因组和进化研究提供基础。
