无间隙的雄性和雌性斑长须鲇染色体水平基因组。

Gap-free chromosome-level genomes of male and female spotted longbarbel catfish Hemibagrus guttatus.

机构信息

Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species, Ministry of Agriculture and Rural Affairs, Guangdong Modern Recreational Fisheries Engineering Technology Center, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.

Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518057, China.

出版信息

Sci Data. 2024 Jun 4;11(1):572. doi: 10.1038/s41597-024-03424-8.

DOI:10.1038/s41597-024-03424-8

PMID:38834584

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11150378/

Abstract

Hemibagrus guttatus, also named as spotted longbarbel catfish, is an economical fish in China. However, their gender cannot be easily distinguished from their appearance, which largely impedes their artificial breeding. Therefore, we provided two gap-free chromosome-level genomes of male and female spotted longbarbel catfish by combining wtdbg2, LR_Gapcloser and TGS-GapCloser assembly approaches with Hi-C data and accurate Pacbio HiFi long-reads. We assembled 30 chromosomes without any gap. Their genome sizes are approximately 749.1 Mb and 747.8 Mb of male and female individuals. The completeness results of BUSCO evaluation show about 94.2% and 95.0%, representing a high-level of completeness of both genomes. We also obtained 35,277 and 34,571 protein-coding gene sets from male and female individuals. Both available gap-free chromosome-level genomes of H. guttatus will provide excellent references for resequencing of male and female individuals to identify accurate markers for distinguishing gender of this fish.

摘要

黄颡鱼，又名斑点叉尾鮰，是中国的一种经济型鱼类。然而，它们的性别很难从外观上区分，这在很大程度上阻碍了它们的人工繁殖。因此，我们通过结合 wtdbg2、LR_Gapcloser 和 TGS-GapCloser 组装方法以及 Hi-C 数据和准确的 Pacbio HiFi 长读，提供了两条无间隙的染色体水平的雄性和雌性斑点叉尾鮰基因组。我们组装了 30 条没有任何间隙的染色体。它们的基因组大小约为 749.1 Mb 和 747.8 Mb 的雄性和雌性个体。BUSCO 评估的完整性结果表明，两者的完整性约为 94.2%和 95.0%，代表了两个基因组的高水平完整性。我们还从雄性和雌性个体中获得了 35,277 个和 34,571 个蛋白质编码基因座。这两条可用的无间隙染色体水平的 H. guttatus 基因组将为雄性和雌性个体的重测序提供极好的参考，以鉴定这种鱼性别区分的准确标记。