Leiva Beatriz, Irastorza Igor, Moneo Andrea, Ibarretxe Gaskon, Silvan Unai, Lanceros-Méndez Senentxu
Basque Centre for Materials, Applications and Nanostructures (BCMaterials), UPV/EHU Science Park, 48940 Leioa, Spain.
Physics Centre of Minho and Porto Universities (CF-UM-UP) and LaPMET-Laboratory of Physics for Materials and Emergent Technologies, University of Minho, 4710-057 Braga, Portugal.
Biomimetics (Basel). 2025 Feb 19;10(2):126. doi: 10.3390/biomimetics10020126.
Polyvinylidene fluoride (PVDF) is a synthetic semicrystalline fluoropolymer with great potential for tissue engineering applications. In addition to its excellent mechanical strength, thermal stability, biocompatibility and simple processability into different morphologies, the relevance of PVDF-based materials for tissue engineering applications comes for its electroactive properties, which include piezo-, pyro- and ferroelectricity. Nevertheless, its synthetic nature and inherent hydrophobicity strongly limit the applicability of this polymer for certain purposes, particularly those involving cell attachment. In addition, the variable adhesion of cells and proteins to PVDF surfaces with different net surface charge makes it difficult to accurately compare the biological response in each case. In this work, we describe a method for the surface functionalization of PVDF films with biological molecules. After an initial chemical modification, and, independently of its polarization state, the PVDF films covalently bind equivalent amounts of cell-binding proteins. In addition, the materials retain their properties, including piezoelectric activity, representing a very promising method for the functionalization of PVDF-based tissue engineering approaches.
聚偏氟乙烯（PVDF）是一种合成半结晶含氟聚合物，在组织工程应用方面具有巨大潜力。除了具有优异的机械强度、热稳定性、生物相容性以及易于加工成不同形态外，基于PVDF的材料在组织工程应用中的相关性还源于其电活性特性，包括压电性、热释电性和铁电性。然而，其合成性质和固有的疏水性严重限制了这种聚合物在某些用途中的适用性，特别是那些涉及细胞附着的用途。此外，细胞和蛋白质对具有不同净表面电荷的PVDF表面的可变粘附性使得难以准确比较每种情况下的生物学反应。在这项工作中，我们描述了一种用生物分子对PVDF膜进行表面功能化的方法。经过初步化学修饰后，无论其极化状态如何，PVDF膜都能共价结合等量的细胞结合蛋白。此外，这些材料保留了它们的特性，包括压电活性，这代表了一种用于基于PVDF的组织工程方法功能化的非常有前景的方法。
