Morris A S, Shearer J, Henry W, Mastrofrancesco B, Caldwell M D
J Surg Res. 1985 Apr;38(4):373-82. doi: 10.1016/0022-4804(85)90051-4.
A marked cellular infiltrate accompanies wounding. The phagocytic and bacteriocidal activities of this infiltrate require increased substrate and O2 consumption. This rapid utilization of available oxygen and substrates could jeopardize an already compromised resident cellular component of a wound. Recent studies have demonstrated macrophage-mediated cell stimulatory agents which induce proliferation of nonlymphoid mesenchymal cells. This study was designed to examine macrophage-resident tissue interactions and their possible significance in wounded tissue. A reconstituted wound system was designed which combines the major components of a lambda-carrageenan skeletal muscle wound (muscle + macrophages). The extensor digitorum longi (EDL) of male Fisher rats were incubated in a standardized fashion. The groups of EDL were muscle incubated alone, muscle with the addition to the incubate of activated peritoneal macrophages or muscle with the addition of a conditioned supernatant from the incubation of activated or nonactivated peritoneal macrophages. Muscle ATP and CP content were noted to be increased 46 and 22%, respectively, when macrophages and skeletal muscle were coincubated. Macrophage-conditioned media from activated or nonactivated macrophages increased the ATP and CP muscle content 44 and 37%, respectively. Preliminary characterization of this high energy phosphate (HEP) promoting factor demonstrates it to be heat and cold stable and less than 10,000 Da. Therefore, a macrophage-mediated transferable factor is capable of increasing the HEP content of skeletal muscle in an in vitro system. This may have important consequences in maintaining host cell integrity following injury.
伤口会伴有明显的细胞浸润。这种浸润的吞噬和杀菌活性需要增加底物和氧气的消耗。对可用氧气和底物的这种快速利用可能会危及伤口中原本就已受损的驻留细胞成分。最近的研究已经证明了巨噬细胞介导的细胞刺激剂可诱导非淋巴间充质细胞增殖。本研究旨在检查巨噬细胞与驻留组织的相互作用及其在受伤组织中的可能意义。设计了一种重组伤口系统，该系统结合了λ-角叉菜胶骨骼肌伤口的主要成分（肌肉+巨噬细胞）。雄性Fisher大鼠的趾长伸肌（EDL）以标准化方式进行孵育。EDL组分别为单独孵育的肌肉、孵育液中添加活化腹膜巨噬细胞的肌肉或添加活化或未活化腹膜巨噬细胞孵育的条件上清液的肌肉。当巨噬细胞和骨骼肌共同孵育时，肌肉ATP和CP含量分别增加了46%和22%。来自活化或未活化巨噬细胞的巨噬细胞条件培养基分别使肌肉ATP和CP含量增加了44%和37%。对这种高能磷酸盐（HEP）促进因子的初步表征表明它对热和冷稳定，分子量小于10,000 Da。因此，一种巨噬细胞介导的可转移因子能够在体外系统中增加骨骼肌的HEP含量。这可能对损伤后维持宿主细胞完整性具有重要意义。
