Amaral J F, Shearer J D, Mastrofrancesco B, Gann D S, Caldwell M D
Surgery. 1986 Aug;100(2):252-61.
The role of lactate in the metabolism of the healing wound is poorly understood. The purpose of the present studies was to determine if despite a net lactate production, wounded (Wx) tissue could metabolize lactate and use it as an oxidative fuel. The extensor digitorum longus muscles (EDL) of weanling, male, Fischer rats were injured with lambda-carrageenan or injured thermally, and 5 and 3 days later, respectively, were incubated in a standard incubate that contained varying amounts of lactate (0 to 6 mmol/L added). Lactate uptake and oxidation, occurred in lambda-carrageenan Wx EDL, thermally injured EDL and non-Wx EDL in a dose-dependent manner. At lactate concentrations of less than 3 mmol/L in the incubate, there was net lactate production, but at lactate concentrations of 6 mmol/L there was no net lactate production by both Wx and non-Wx EDL. The increase in lactate oxidation was not associated with an alteration in the tissue content of adenosine triphosphate or creatine phosphate. It was associated with a reduction in glucose oxidation in Wx and non-Wx EDL and by a decrease in glucose uptake by Wx EDL. These data suggest that lactate may be used as an oxidative fuel by wounded tissue and in this regard may substitute for glucose.
乳酸在愈合伤口代谢中的作用目前还知之甚少。本研究的目的是确定尽管存在乳酸净生成，但受伤（Wx）组织是否能够代谢乳酸并将其用作氧化燃料。用λ-角叉菜胶或热损伤断奶雄性Fischer大鼠的趾长伸肌（EDL），分别在5天和3天后，将其置于含有不同量乳酸（添加量为0至6 mmol/L）的标准培养液中孵育。乳酸摄取和氧化在λ-角叉菜胶处理的Wx EDL、热损伤的EDL和未受伤的EDL中呈剂量依赖性发生。在培养液中乳酸浓度低于3 mmol/L时，存在乳酸净生成，但在乳酸浓度为6 mmol/L时，Wx和未受伤的EDL均未出现乳酸净生成。乳酸氧化的增加与三磷酸腺苷或磷酸肌酸的组织含量变化无关。它与Wx和未受伤的EDL中葡萄糖氧化的减少以及Wx EDL对葡萄糖摄取的减少有关。这些数据表明，受伤组织可能将乳酸用作氧化燃料，在这方面可能替代葡萄糖。
