Terakawa N, Aono T, Tsutsumi H, Matsumoto K
J Steroid Biochem. 1985 Apr;22(4):475-80. doi: 10.1016/0022-4731(85)90165-7.
Replenishment of uterine estrogen receptor (ER) following a single injection of estradiol-17 beta (E2) was examined in chronically estrogenized rats. Subcutaneous implantation of E2-pellet for 7 days in ovariectomized rats resulted in a significant stimulation of uteri with regard to wet tissue weight, DNA content and progesterone receptor content, with a shift of ER distribution. An intraperitoneal injection of 5 micrograms E2 in the E2-implanted rats induced a significant decrease in soluble ER (from 141.1 +/- 12.6 to 69.2 +/- 8.8 fmol/mg protein) with a concomitant increase in nuclear ER (from 58.2 +/- 8.6 to 129.2 +/- 11.6 fmol/100 micrograms DNA) 1 h after the injection. However, soluble ER was rapidly replenished, which was accompanied by nuclear ER reduction, and both values returned to the pre-injection levels at 4 h after the injection. An administration of 150 micrograms cycloheximide, that effectively blocked protein synthesis in the uterus of the E2-implanted rats, completely inhibited the replenishment of soluble ER induced by 5 micrograms E2. These findings, combined with our previous findings that replenishment of ER following a single E2 administration in the pituitary of chronically estrogenized rats was inhibited by cycloheximide, suggest that replenishment of ER is entirely dependent on protein synthesis in chronically estrogenized rats.
在长期雌激素化的大鼠中，研究了单次注射雌二醇 - 17β（E2）后子宫雌激素受体（ER）的补充情况。在去卵巢大鼠中皮下植入E2丸剂7天，导致子宫在湿组织重量、DNA含量和孕激素受体含量方面受到显著刺激，同时ER分布发生改变。给植入E2的大鼠腹腔注射5微克E2，注射后1小时可溶性ER显著降低（从141.1±12.6降至69.2±8.8 fmol/mg蛋白质），同时核ER增加（从58.2±8.6升至129.2±11.6 fmol/100微克DNA）。然而，可溶性ER迅速得到补充，同时核ER减少，注射后4小时两者的值均恢复到注射前水平。给予150微克环己酰亚胺可有效阻断植入E2大鼠子宫中的蛋白质合成，完全抑制5微克E2诱导的可溶性ER的补充。这些发现，结合我们之前的发现，即在长期雌激素化大鼠的垂体中单次给予E2后ER的补充受到环己酰亚胺的抑制，表明在长期雌激素化大鼠中ER的补充完全依赖于蛋白质合成。
