Sato B, Nishizawa Y, Noma K, Kishimoto S, Matsumoto K
Biochim Biophys Acta. 1983 Feb 22;755(3):412-9. doi: 10.1016/0304-4165(83)90244-1.
Estrogen receptor content and dynamics in the uteri obtained from chronically estrogenized rats were analyzed. 12 day treatment with a subcutaneous implantation of a diethylstilbestrol pellet resulted in maximal stimulation of uteri with regard to wet tissue weight, DNA content, as well as progesterone receptor content without significant alteration of the estrogen receptor level. Estrogen receptor dynamics in just ovariectomized or ovariectomized and diethylstilbestrol-stimulated rats elicited by a single injection of estradiol were next examined using the exchange methods. The cytosol receptor content rapidly declined, with a small and temporary accumulation of the nuclear receptor in the uterus from rats continuously exposed to diethylstilbestrol during the preceding 12 days. A relatively rapid cytosol receptor replenishment was also observed in rats pretreated with diethylstilbestrol. This was accompanied by a rapid decrease in the nuclear receptor level to 70% of the preinjection value at 5 h after estradiol administration. These data are in contrast to findings on uteri of ovariectomized and nonestrogen-treated rats, in which a single injection of estradiol resulted in a prolonged nuclear receptor retention and a delayed cytosol receptor replenishment. Adrenalectomy did not result in a significant change of receptor dynamic patterns, suggesting that adrenal steroids do not play a role in the alteration of receptor dynamics elicited by continuous stimulation with diethylstilbestrol. These observations suggest that a continuous exposure of rat uteri to the estrogen causes an altered regulation of estrogen receptor dynamics by the homologous steroid compared to those in chronically estrogen-deprived rats.
对从长期雌激素化大鼠获得的子宫中的雌激素受体含量和动态变化进行了分析。皮下植入己烯雌酚丸粒进行12天的处理，导致子宫在湿组织重量、DNA含量以及孕酮受体含量方面受到最大程度的刺激，而雌激素受体水平没有显著改变。接下来，使用交换方法检查了仅接受卵巢切除术或卵巢切除术后并用己烯雌酚刺激的大鼠在单次注射雌二醇后引发的雌激素受体动态变化。在先前12天持续暴露于己烯雌酚的大鼠子宫中，胞质溶胶受体含量迅速下降，同时核受体有少量且短暂的积累。在用己烯雌酚预处理的大鼠中也观察到相对快速的胞质溶胶受体补充。这伴随着在注射雌二醇后5小时核受体水平迅速下降至注射前值的70%。这些数据与卵巢切除且未接受雌激素处理的大鼠子宫的研究结果形成对比，在这些大鼠中，单次注射雌二醇会导致核受体长时间保留以及胞质溶胶受体补充延迟。肾上腺切除术并未导致受体动态模式发生显著变化，这表明肾上腺类固醇在己烯雌酚持续刺激引起的受体动态变化中不起作用。这些观察结果表明，与长期雌激素缺乏的大鼠相比，大鼠子宫持续暴露于雌激素会导致同源类固醇对雌激素受体动态变化的调节发生改变。
