Nagele R G, Goldstein M M, Lee H
J Microsc. 1979 Jul;116(2):287-90. doi: 10.1111/j.1365-2818.1979.tb00212.x.
A method is described for processing multiple cross-sections from early chick embryos for scanning electron microscopy. Embryos are cut through the desired regions. Sections are affixed to a coverslip with Duco cement and critical point dried by Freon 13 or liquid CO2. This method provides a reliable means for preparing multiple cross-sections from single embryos and eliminates the need for direct handling of brittle tissues after drying.
