Weber D F, Eisenmann D R, El-Moneim Zaki A
J Microsc. 1982 Sep;127(Pt 3):271-6. doi: 10.1111/j.1365-2818.1982.tb00422.x.
We have developed a method utilizing relatively thick ground sections of plastic embedded tissue which affords the resolution obtained with 0.5 micrometers cut sections. The sections, which are permanently affixed to plastic microscope slides, are much larger in area than ultramicrotome sections. Additional advantages are: sections can be destained and restained and selected areas can be examined with various forms of electron microscopy. Autoradiographic studies are also possible. Although the method has a broader application, it is particularly useful in examining the interface between hard and soft tissues.
我们已经开发出一种方法，该方法利用塑料包埋组织的相对较厚的地面切片，可提供与0.5微米厚切片相当的分辨率。这些切片永久固定在塑料显微镜载玻片上，其面积比超薄切片大得多。其他优点包括：切片可以脱色和重新染色，选定区域可以用各种形式的电子显微镜进行检查。放射自显影研究也是可行的。尽管该方法有更广泛的应用，但它在检查硬组织和软组织之间的界面时特别有用。
