Fu Qin, Bai Bin, Zhou Sitong, Zhao Yunlong, Yang Yue, Zhang Xiaohong, Liu Xuanning, Wu Mengyao, Zhao Wenzhong, Bao Jun, Liu Honggui
College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang, P.R. China.
Institute of New Rural Development, Harbin, Heilongjiang, P.R. China.
PLoS One. 2025 Feb 25;20(2):e0313354. doi: 10.1371/journal.pone.0313354. eCollection 2025.
The purpose of this study was to explore whether music can reduce stress in animals by regulating the activity of the hypothalamus pituitary adrenal cortex (HPA) axis and reducing the concentration of cortisol. The control group was not played with any music or mechanical noise, the music group played music before and during transport, and the noise group played noise before and during transport as a positive control. The results showed that after two-hours of transportation, the concentrations of adrenocorticotropic hormone and cortisol in the music group were lower than that in the control and the noise groups, while the cortisol level in the noise group was higher than that in the music and the control groups. Plasma concentrations of D-lactic acid and diamine oxidase in the music group were lower than those in the control group and the noise group, and the noise group was higher than the control group. In addition, the concentrations of jejunal inflammatory factors interleukin-6 and interleukin-8 in the music group were lower than those in the control group and the noise group, but there was no difference of interleukin-12 in the three groups. However, there was no significant difference in Illinois-6, Illinois-12 and INF-γ between the noise group and the control group. The contents of reactive oxygen species, malondialdehyde and glutathione peroxidase in the music group had no changes compared with those in the control group, while the contents of reactive oxygen species and malondialdehyde in the noise group were higher than the control and the music groups, and the content of glutathione peroxidase was decreased. Compared with the music group, differently expressed genes analysis also showed that the mRNA expression level of inflammatory genes in the jejunum of the music group' piglets decreased. In addition, compared with the music group, some Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to inflammation were highly expressed in the control group. In conclusion, our results showed that musical stimulation can reduce the inflammatory response of piglets caused by transportation by reducing the activity of HPA axis. Noise increased the activity of HPA axis, which aggravated the intestinal damage of piglets and caused intestinal oxidative damage.
本研究的目的是探讨音乐是否能通过调节下丘脑 - 垂体 - 肾上腺皮质（HPA）轴的活性并降低皮质醇浓度来减轻动物的应激反应。对照组不播放任何音乐或机械噪音，音乐组在运输前和运输过程中播放音乐，噪音组在运输前和运输过程中播放噪音作为阳性对照。结果显示，运输两小时后，音乐组促肾上腺皮质激素和皮质醇的浓度低于对照组和噪音组，而噪音组的皮质醇水平高于音乐组和对照组。音乐组血浆中D - 乳酸和二胺氧化酶的浓度低于对照组和噪音组，且噪音组高于对照组。此外，音乐组空肠炎症因子白细胞介素 - 6和白细胞介素 - 8的浓度低于对照组和噪音组，但三组间白细胞介素 - 12无差异。然而，噪音组与对照组之间白细胞介素 - 6、白细胞介素 - 12和干扰素 - γ无显著差异。音乐组活性氧、丙二醛和谷胱甘肽过氧化物酶的含量与对照组相比无变化，而噪音组活性氧和丙二醛的含量高于对照组和音乐组，谷胱甘肽过氧化物酶的含量降低。与音乐组相比，差异表达基因分析还显示音乐组仔猪空肠中炎症基因的mRNA表达水平降低。此外，与音乐组相比，对照组中一些与炎症相关的京都基因与基因组百科全书（KEGG）通路高表达。总之，我们的结果表明，音乐刺激可通过降低HPA轴的活性来减轻运输引起的仔猪炎症反应。噪音增加了HPA轴的活性，加重了仔猪的肠道损伤并导致肠道氧化损伤。
