锌指蛋白2抑制拟南芥珠柄木质化以确保种子装载效率。

ZINC FINGER PROTEIN2 suppresses funiculus lignification to ensure seed loading efficiency in Arabidopsis.

作者信息

Low Pui Man, Kong Que, Blaschek Leonard, Ma Zhiming, Lim Peng Ken, Yang Yuzhou, Quek Trisha, Lim Cuithbert J R, Singh Sanjay K, Crocoll Christoph, Engquist Ellen, Thorsen Jakob S, Pattanaik Sitakanta, Tee Wan Ting, Mutwil Marek, Miao Yansong, Yuan Ling, Xu Deyang, Persson Staffan, Ma Wei

机构信息

School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.

Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark.

出版信息

Dev Cell. 2025 Jun 23;60(12):1719-1729.e6. doi: 10.1016/j.devcel.2025.01.021. Epub 2025 Feb 24.

DOI:10.1016/j.devcel.2025.01.021

PMID:39999844

Abstract

The plant funiculus anchors the developing seed to the placenta within the inner dorsal pod strands of the silique wall and directly transports nutrients to the seeds. The lignified vasculature critically supports nutrient transport through the funiculus. However, molecular mechanisms underlying lignified secondary cell wall (SCW) biosynthesis in the funiculus remain elusive. Here, we show that the transcription factor ZINC FINGER PROTEIN2 (ZFP2) represses SCW formation in the cortex cells that surround the vasculature. This function is essential for efficient nutrient loading into the seeds. Notably, ZFP2 directly acts on the SCW transcription factor NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1) to repress cortex cell lignification, providing a mechanism of how SCW biosynthesis is restricted to the vasculature of the funiculus to ensure proper seed loading in Arabidopsis.

摘要

植物珠柄将发育中的种子固定在角果壁内背侧荚果束中的胎座上，并直接将营养物质运输到种子中。木质化的维管系统对通过珠柄的营养物质运输起着关键的支持作用。然而，珠柄中木质化次生细胞壁（SCW）生物合成的分子机制仍然不清楚。在这里，我们表明转录因子锌指蛋白2（ZFP2）抑制围绕维管系统的皮层细胞中SCW的形成。该功能对于将营养物质有效装载到种子中至关重要。值得注意的是，ZFP2直接作用于SCW转录因子NAC次生壁增厚促进因子1（NST1）以抑制皮层细胞木质化，这为SCW生物合成如何局限于珠柄的维管系统以确保拟南芥中种子的正常装载提供了一种机制。