Fleming B P, McKinney M E
Microvasc Res. 1985 May;29(3):387-400. doi: 10.1016/0026-2862(85)90027-5.
The distribution of adrenergic nerves to the microcirculation of the wing web of the bat was studied in whole-mount preparations employing glyoxylic acid fluorescence histochemistry. A dense perivascular adrenergic nerve plexus consisting of various-sized nerve bundles surrounded the arteries. The accompanying veins were sparsely innervated by small nerve bundles or single axons. Arterioles also possessed a dense perivascular plexus comprised of a loose network of small axon bundles. Muscular venules accompanying most arterioles were supplied by a small number of varicose axons which surrounded these vessels in a loose network. The majority of the terminal arterioles were devoid of any direct innervation. The proximal segment of some terminal arterioles was innervated by one or two axons which were observed to diverge from the vessels and terminate in the tissue. The true capillaries, postcapillary venules, and lymphatics possessed no direct adrenergic innervation. Surgical sectioning of the appropriate nerve trunks or administration of 6-hydroxydopamine caused complete disappearance of all fluorescent adrenergic axons in the wing web. Faint acetylcholinesterase-positive staining was observed in a perivascular plexus surrounding the arteries and arterioles. A much diminished acetylcholinesterase activity was observed after treatment with 6-hydroxydopamine which implies that this enzymatic activity resides in the adrenergic nerves.
利用乙醛酸荧光组织化学技术，在整装标本中研究了肾上腺素能神经在蝙蝠翼膜微循环中的分布。由各种大小神经束组成的致密血管周围肾上腺素能神经丛环绕着动脉。伴随的静脉由小神经束或单个轴突稀疏地支配。微动脉也有一个由小轴突束松散网络组成的致密血管周围丛。大多数微动脉伴随的肌性微静脉由少量曲张轴突供应，这些轴突以松散网络形式围绕这些血管。大多数终末微动脉没有任何直接神经支配。一些终末微动脉的近端段由一两个轴突支配，这些轴突从血管分出并终止于组织中。真正的毛细血管、毛细血管后微静脉和淋巴管没有直接的肾上腺素能神经支配。对适当神经干进行手术切断或给予6-羟基多巴胺会导致翼膜中所有荧光肾上腺素能轴突完全消失。在动脉和微动脉周围的血管周围丛中观察到微弱的乙酰胆碱酯酶阳性染色。用6-羟基多巴胺处理后，乙酰胆碱酯酶活性明显降低，这表明这种酶活性存在于肾上腺素能神经中。
