Barajas L, Wang P
Anat Rec. 1979 Nov;195(3):525-34. doi: 10.1002/ar.1091950311.
The innervation of the glomerular arterioles was investigated by light and electron microscopy autoradiography for localization of exogenous tritiated norepinephrine. By light microscopy accumulations of grains were seen associated with afferent arterioles and in lesser numbers with efferent arterioles and neighboring tubules. Accumulations of grains were noted to be in contact with juxtaglomerular granular cells. Electron microscopy autoradiography revealed that nearly two-thirds of the silver grains were on axons. Most of the label was on varicosities packed with small, clear and dense-cored, vesicles. Most varicosities, including those in contact with smooth muscle, juxtaglomerular granular or tubular cells, were labeled. Some varicosities which appeared unlabeled in a given section were labeled in subsequent sections. These findings are consistent with the notion that the glomerular arterioles are innervated mainly by adrenergic nerves. This view is supported by the previously reported observations of the concomitant virtual disappearance of fluorescent and acetylcholinesterase-positive nerves from the region of the glomerular arterioles after two injections of six-hydroxydoapmine (a drug which selectively destroys adrenergic nerves) and the presence of small dense-cored vesicles in all axons of the juxtaglomerular region when examined by serial section electron microscopy.
通过光学显微镜和电子显微镜放射自显影术研究肾小球小动脉的神经支配，以定位外源性氚标记去甲肾上腺素。光学显微镜下可见与入球小动脉相关的银粒聚集，而出球小动脉和邻近肾小管的银粒聚集较少。注意到银粒聚集与球旁颗粒细胞接触。电子显微镜放射自显影显示，近三分之二的银粒位于轴突上。大多数标记位于充满小的、清亮和致密核心小泡的膨体上。大多数膨体，包括那些与平滑肌、球旁颗粒细胞或肾小管细胞接触的膨体，都有标记。在给定切片中看似未标记的一些膨体在后续切片中有标记。这些发现与肾小球小动脉主要由肾上腺素能神经支配的观点一致。这一观点得到了先前报道的观察结果的支持，即两次注射6-羟基多巴胺（一种选择性破坏肾上腺素能神经的药物）后，肾小球小动脉区域的荧光和乙酰胆碱酯酶阳性神经几乎同时消失，并且通过连续切片电子显微镜检查发现球旁区域的所有轴突中都存在小的致密核心小泡。
