Wang Qianqian, Yuan Yi, Yan Jiaqing
School of Electrical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, P. R. China.
Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province, Yanshan University, Qinhuangdao, Hebei 066004, P. R. China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2024 Dec 25;41(6):1161-1168. doi: 10.7507/1001-5515.202401047.
Low-intensity ultrasound stimulation of the retina has the ability to modulate neural activity in the primary visual cortex (V1), however, it is currently unclear how different intensities and durations of ultrasonic stimulation of the retina modulate neural activity in V1. In this paper, we recorded local field potential (LFP) signals in the V1 brain region of mice under different ultrasound intensities and different stimulation times. The amplitude of LFP corresponding to 1 s before ultrasound stimulation to 2 s after stimulation (-1-2 s) was analyzed, including the power and sample entropy of delta, theta, alpha beta, and low gamma frequency bands. The experimental results showed that, as the stimulation intensity increased, the peak value of the LFP in the visual cortex showed a linear upward trend; the power in the delta and theta frequency bands showed a linear upward trend, and the sample entropy showed a linear downward trend. With increases of stimulation duration, the peak value of the LFP in the visual cortex showed an upward trend, and the upward trend gradually weakened; the power in the delta frequency band showed an upward trend, the sample entropy showed a linear upward trend, and the sample entropy in the theta frequency band showed a downward trend. The results show that low-intensity ultrasonic stimulation of the retina has a significant modulatory effect on neural activity in the visual cortex. The study provides insights into the mechanisms by which ultrasonic stimulation regulates visual system function. Furthermore, it clarifies the patterns of parameter selection, facilitating the development of personalized multi-parameter modulation for the treatment of visual neural degeneration, retinal disorders and related research areas.
低强度超声刺激视网膜能够调节初级视觉皮层（V1）的神经活动，然而，目前尚不清楚不同强度和持续时间的视网膜超声刺激如何调节V1区的神经活动。在本文中，我们记录了不同超声强度和不同刺激时间下小鼠V1脑区的局部场电位（LFP）信号。分析了超声刺激前1秒至刺激后2秒（-1至2秒）对应的LFP幅度，包括δ、θ、αβ和低γ频段的功率和样本熵。实验结果表明，随着刺激强度的增加，视觉皮层中LFP的峰值呈线性上升趋势；δ和θ频段的功率呈线性上升趋势，样本熵呈线性下降趋势。随着刺激持续时间的增加，视觉皮层中LFP的峰值呈上升趋势，且上升趋势逐渐减弱；δ频段的功率呈上升趋势，样本熵呈线性上升趋势，θ频段的样本熵呈下降趋势。结果表明，低强度超声刺激视网膜对视觉皮层的神经活动具有显著的调节作用。该研究为超声刺激调节视觉系统功能的机制提供了见解。此外，它阐明了参数选择模式，有助于开发个性化的多参数调制方法用于治疗视觉神经退行性变、视网膜疾病及相关研究领域。
